p85α/p110α Protein, Human (Active, E545K, Biotinylated, sf9, Avi)
Based on 1 Customer Validation
p85/p110 Protein, Human (Active, E545K, Biotinylated, sf9, Avi) is the recombinant human-derived p85/p110, expressed by sf9 insect cells , with Avi labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
p85/p110 Protein, Human (Active, E545K, Biotinylated, sf9, Avi) is the recombinant human-derived p85/p110, expressed by sf9 insect cells , with Avi labeled tag. ,
Verified Bioactivity
The activity of p85/p110 is verified by the ADP-Glo method: Incubate p85/p110 protein, ATP, and the substrate at room temperature for 40 minutes, then add the ADP-Glo reagent and read the luminescence signal using the chemiluminescence module of the microplate reader.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-Avi
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Accession
P27986-1 (A318-D615)&P42336 (P2-N1068, E545K)
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Gene ID5295&5290
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Conjugation
Biotin
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Synonyms
PIK3R1; PI3-Kinase Subunit P85-Alpha; Phosphoinositide-3-Kinase Regulatory Subunit 1; PI3 Kinase-Associated P85; GRB1; CDNA FLJ44373 Fis, Clone TRACH3009455, Highly Similar To Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha; Phosphatidylinositol 3-Kinase 85 KDa Regulatory Subunit Alpha; Phosphatidylinositol 3-Kinase, Regulatory Subunit, Polypeptide 1 (P85 Alpha); Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha; Phosphatidylinositol 3-Kinase-Associated P-85 Alpha; P85-ALPHA; PtdIns-3-Kinase Regulatory Subunit P85-Alpha; P85alpha; Phosphoinositide-3-Kinase Regulatory Subunit; P85; PI3-Kinase Regulatory Subunit Alpha; Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha); PIK3R1/NR4A3 Fusion Protein; Phosphoinositide-3-Kinase Regulatory Subunit Alpha; NR4A3 Fusion; PtdIns-3-Kinase Regulatory Subunit Alpha; IMD36; Growth Factor Receptor Bound 1; AGM7; PI3K Regulatory Subunit Alpha; MHC class I antigen; MHC class I protein; Mamu-A1; Mamu-A
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Predicted Molecular Mass
162.8 kDa
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Molecular Weight
Approximately 130-150 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.2 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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