PCSK1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The PCSK1 protein is a proprotein convertase that plays a key role in processing hormone and protein precursors at sites with basic amino acid residues. It targets multiple substrates such as POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP. PCSK1 Protein, Human (HEK293, His) is the recombinant human-derived PCSK1 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The PCSK1 protein is a proprotein convertase that plays a key role in processing hormone and protein precursors at sites with basic amino acid residues. It targets multiple substrates such as POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP. PCSK1 Protein, Human (HEK293, His) is the recombinant human-derived PCSK1 protein, expressed by HEK293 , with C-His labeled tag.
Background
The PCSK1 Protein plays a pivotal role in the processing of hormone and other protein precursors, particularly at sites characterized by pairs of basic amino acid residues. Functioning as a proprotein convertase, PCSK1 targets a range of substrates, including POMC, renin, enkephalin, dynorphin, somatostatin, insulin, and AGRP. Through its enzymatic activity, PCSK1 is involved in the proteolytic cleavage and maturation of these precursor molecules, thereby regulating the generation of active hormones and neuropeptides. The diverse substrates highlight the broad impact of PCSK1 on the processing of proteins crucial for various physiological functions, including metabolism, endocrine signaling, and neuropeptide regulation.
Verified Bioactivity
Measured by its ability to cleave the fluorogenic peptide 200 µM substrate pERTKR-AMC. that incubate at 37°C temperature for 60 minutes. The specific activity is 116.38 pmol/min/µg.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-10*His
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Accession
P29120-1/NP_000430.3 (S111-R617)
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Molecular Construction
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N-term
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PCSK1 (S111-R617)
Accession # P29120-1/NP_000430.3 -
His
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C-term
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Protein Length
Partial
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Synonyms
PCSK1; SPC3; Prev. NEC1; Proprotein Convertase 1/3; Neuroendocrine Convertase 1; Prohormone Convertase 3; Prohormone Convertase 1; Proprotein Convertase 1; PC1; BMIQ12; PC1/3; NEC 1; Proprotein Convertase Subtilisin/Kexin Type 1; PC3
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AA Sequence
SALRDSALNLFNDPMWNQQWYLQDTRMTAALPKLDLHVIPVWQKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAMQANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKTVEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIFALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRSVPEKKECVVKDNDFEPRALKANGEVIIEIPTRACEGQENAIKSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGTWTLRITDMSGRIQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDR
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Molecular Weight
Approximately 66 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 25 mM Tris-HCl, 150 mM NaCl, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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