PDE1C Protein, Human (sf9, His-GST)
PDE1C, a member of the phosphodiesterase (PDE) family, is a subtype of PDE activated by Ca2+.PDE1 plays an important role in the cardiovascular system.PDE inhibitors can reduce neuroinflammation and oxidative stress, stimulate neuroplasticity, and improve symptoms of mental disorders in preclinical models by activating the CAMP-PGKA-CREB and cGMP-PKG pathways.PDE1C Protein, Human (sf9, His-GST) is the recombinant human-derived PDE1C protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
PDE1C, a member of the phosphodiesterase (PDE) family, is a subtype of PDE activated by Ca2+.PDE1 plays an important role in the cardiovascular system.PDE inhibitors can reduce neuroinflammation and oxidative stress, stimulate neuroplasticity, and improve symptoms of mental disorders in preclinical models by activating the CAMP-PGKA-CREB and cGMP-PKG pathways.PDE1C Protein, Human (sf9, His-GST) is the recombinant human-derived PDE1C protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
Phosphodiesterase (PDE) is an enzyme that breaks phosphodiester bonds, usually ring nucleotide phosphodiesterase. Phosphodiesterases consist of a group of enzymes that degrade the phosphodiester bonds in the second messenger molecule cAMP and cGMP. They regulate the localization, duration, and amplitude of cyclic nucleotide signals within subcellular domains. PDE inhibitors can reduce neuroinflammation and oxidative stress, stimulate neuroplasticity, and improve symptoms of mental disorders in preclinical models by activating the CAMP-PGKA-CREB and cGMP-PKG pathways. PDE1 is composed of three isomers: PDE1A, PDE1B and PDE1C. PDE1 is the only known subtype of PDE activated by Ca2+. PDE1 plays an important role in the cardiovascular system[1][2][3].
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
Q8TAE4 (M1-E634)
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Gene ID/
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Molecular Construction
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N-term
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His-GST
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PDE1C (M1-E634)
Accession # Q8TAE4 -
C-term
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Protein Length
Full Length
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Synonyms
PDE1C; Phosphodiesterase 1C, Calmodulin-Dependent (70kD); Phosphodiesterase 1C; 3',5'-Cyclic-GMP Phosphodiesterase; Hcam3; 3',5'-Cyclic-AMP Phosphodiesterase; Dual Specificity Calcium/Calmodulin-Dependent 3',5'-Cyclic Nucleotide Phosphodiesterase 1C; Unch
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AA Sequence
MESPTKEIEEFESNSLKYLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQLERGEASVVDLKKNLEYAATVLESVYIDETRRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFVERMYRRTSNMVGLSYPPAVIEALKDVDKWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALVSFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFHIQTRSDPAILYNDRSVLENHHLSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIKAMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKSTMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEGSAPINNSVISVDYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARMAAEEQQKEMEAKSQAEEGASGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQQQNGDFKDGKNKTDKKDHSNIGNDSKKTDDSQE
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Predicted Molecular Mass
100 kDa
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Molecular Weight
Approximately 97 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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