PDGF R beta Protein, Human (Active, Biotinylated, sf9, His, Avi)

PDGF Rβ protein is integral in embryonic development and regulates cell proliferation, survival, differentiation, chemotaxis, migration, and vascular development. It crucially recruits pericytes and smooth muscle cells to the endothelium, promoting vascular smooth muscle cell migration and neointimal formation at the site of injury. PDGF R beta Protein, Human (Biotinylated, sf9, His, Avi) is the recombinant human-derived PDGF R beta, expressed by Sf9 insect cells, with Avi, His labeled tag.

The receptor described in the text is the platelet-derived growth factor receptor alpha (PDGFRA). PDGFRA is involved in various cellular processes, including embryonic development, cell proliferation, survival, differentiation, chemotaxis, migration, and blood vessel development. It plays a crucial role in the recruitment of pericytes and smooth muscle cells to endothelial cells, as well as in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. PDGFRA is required for normal development of the cardiovascular system and for the formation of a branched network of capillaries in kidney glomeruli. It promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its ligands, including homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB, or homodimeric PDGFD, leads to the activation of several signaling cascades. PDGFRA phosphorylates various downstream effectors, including PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1, NCK1, PDCD6IP/ALIX, and STAM. The receptor signaling is regulated by protein phosphatases that dephosphorylate the receptor and its downstream effectors, as well as by the rapid internalization of the activated receptor.

Human

Sf9 insect cells

N-Avi;N-His

P09619-1 (Q557-L1106)

5159

Cytoplasmic Domain

Biotin

QKKPRYEIRWKVIESVSSDGHEYIYVDPMQLPYDSTWELPRDQLVLGRTLGSGAFGQVVEATAHGLSHSQATMKVAVKMLKSTARSSEKQALMSELKIMSHLGPHLNVVNLLGACTKGGPIYIITEYCRYGDLVDYLHRNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGKLVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFTLGGTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRSDHPAILRSQARLPGFHGLRSPLDTSSVLYTAVQPNEGDNDYIIPLPDPKPEVADEGPLEGSPSLASSTLNEVNTSSTISCDSPLEPQDEPEPEPQLELQVEPEPELEQLPDSGCPAPRAEAEDSFL

≥ 80%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.