PDIA6 Protein, Human (HEK293, His)

PDIA6 protein has molecular chaperone activity, which can inhibit the aggregation of misfolded proteins and contribute to quality control. It negatively regulates the unfolded protein response (UPR) by binding to ERN1 and inhibiting its signaling. PDIA6 Protein, Human (HEK293, His) is the recombinant human-derived PDIA6 protein, expressed by HEK293 , with C-6*His labeled tag.

The PDIA6 protein exhibits diverse functions, acting as a chaperone that inhibits the aggregation of misfolded proteins, thereby contributing to protein quality control. Moreover, it negatively regulates the unfolded protein response (UPR) by binding to UPR sensors like ERN1, leading to the inactivation of ERN1 signaling. Additionally, PDIA6 may modulate the UPR through the EIF2AK3 UPR sensor. Beyond its role in protein homeostasis, PDIA6 plays a significant role in platelet aggregation and activation induced by various agonists, such as convulxin, collagen, and thrombin. These multifaceted functions underscore the importance of PDIA6 in cellular processes related to protein folding, quality control, and platelet activation. Further research is needed to unravel the specific molecular mechanisms underlying these diverse functions, providing insights into the broader regulatory roles of PDIA6 in cellular physiology.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

Q15084 (L20-L440)

10130  [NCBI]

Full Length of Mature Protein

LYSSSDDVIELTPSNFNREVIQSDSLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVDADKHHSLGGQYGVQGFPTIKIFGSNKNRPEDYQGGRTGEAIVDAALSALRQLVKDRLGGRSGGYSSGKQGRSDSSSKKDVIELTDDSFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAASEVKEQTKGKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDVELDDLGKDEL

Approximately 52.0 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.