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  4. PDILT Protein, Human (HEK293, His)

PDILT Protein, Human (HEK293, His)

Cat. No.: HY-P71194
Handling Instructions

The PDILT protein is a possible redox-inactive chaperone essential in spermatogenesis, forming non-disulfide-linked homodimers. Its role emphasizes its importance in male germ cell development. PDILT Protein, Human (HEK293, His) is the recombinant human-derived PDILT protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PDILT Protein, Human (HEK293, His) is 564 a.a., with molecular weight of ~92.0 kDa.

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Description

The PDILT protein is a possible redox-inactive chaperone essential in spermatogenesis, forming non-disulfide-linked homodimers. Its role emphasizes its importance in male germ cell development. PDILT Protein, Human (HEK293, His) is the recombinant human-derived PDILT protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PDILT Protein, Human (HEK293, His) is 564 a.a., with molecular weight of ~92.0 kDa.
Background

The PDILT protein is identified as a probable redox-inactive chaperone with involvement in spermatogenesis. Existing as a homodimer, this chaperone does not form disulfide linkages within the dimeric structure. Its role in spermatogenesis underscores its significance in the intricate processes associated with male germ cell development. Furthermore, PDILT interacts with ERO1A and CLGN, indicating potential associations with proteins involved in redox regulation and chaperone activities. A deeper exploration of the specific molecular mechanisms and functional implications of PDILT in spermatogenesis and its interactions with ERO1A and CLGN will contribute to a comprehensive understanding of its role in male reproductive biology.
Species

Human
Source

HEK293
Tag

C-6*His
Accession

Q8N807 (S21-L584)
Gene ID

204474  [NCBI]
Molecular Construction
N-term
PDILT (S21-L584)
Accession # Q8N807
6*His
C-term
Synonyms
Protein Disulfide-Isomerase-Like Protein of the Testis; PDILT
AA Sequence

SPEVNAGVSSIHITKPVHILEERSLLVLTPAGLTQMLNQTRFLMVLFHNPSSKQSRNLAEELGKAVEIMGKGKNGIGFGKVDITIEKELQQEFGITKAPELKLFFEGNRSEPISCKGVVESAALVVWLRRQISQKAFLFNSSEQVAEFVISRPLVIVGFFQDLEEEVAELFYDVIKDFPELTFGVITIGNVIGRFHVTLDSVLVFKKGKIVNRQKLINDSTNKQELNRVIKQHLTDFVIEYNTENKDLISELHIMSHMLLFVSKSSESYGIIIQHYKLASKEFQNKILFILVDADEPRNGRVFKYFRVTEVDIPSVQILNLSSDARYKMPSDDITYESLKKFGRSFLSKNATKHQSSEEIPKYWDQGLVKQLVGKNFNVVVFDKEKDVFVMFYAPWSKKCKMLFPLLEELGRKYQNHSTIIIAKIDVTANDIQLMYLDRYPFFRLFPSGSQQAVLYKGEHTLKGFSDFLESHIKTKIEDEDELLSVEQNEVIEEEVLAEEKEVPMMRKGLPEQQSPELENMTKYVSKLEEPAGKKKTSEEVVVVVAKPKGPPVQKKKPKVKEEL

Molecular Weight

Approximately 92.0 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

PDILT Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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