PDK3 Protein, Human (Inactive, sf9, His, GST)
Based on 1 Customer Validation
PDK3 Protein, Human (Inactive, sf9, His, GST) is the recombinant human-derived PDK3, expressed by sf9 insect cells , with His, GST labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
PDK3 Protein, Human (Inactive, sf9, His, GST) is the recombinant human-derived PDK3, expressed by sf9 insect cells , with His, GST labeled tag. ,
Background
PDK3, a pivotal player in metabolic regulation, exerts its influence by inhibiting pyruvate dehydrogenase activity through the phosphorylation of the E1 subunit PDHA1, thereby finely tuning glucose metabolism and aerobic respiration. Its capacity to phosphorylate PDHA2 adds another layer of control to these critical cellular processes. Under conditions of prolonged fasting or in response to a high-fat diet, PDK3 orchestrates a metabolic shift, reducing glucose utilization and enhancing fat metabolism. Beyond its role in nutrient adaptation, PDK3 emerges as a key contributor to glucose homeostasis, playing a crucial part in maintaining normal blood glucose levels. Additionally, its involvement in the generation of reactive oxygen species adds a layer of complexity to its multifaceted functions.
Verified Bioactivity
The product demonstrated no detectable kinase activity in an ATP/ADP-based kinase assay.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag His;GST
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Accession
Q15120-1 (R2-Q406)
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Gene ID5165
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
PDK3; Pyruvate Dehydrogenase, Lipoamide, Kinase Isozyme 3, Mitochondrial; Pyruvate Dehydrogenase Kinase 3; Pyruvate Dehydrogenase Kinase Isoform 3; [Pyruvate Dehydrogenase (Acetyl-Transferring)] Kinase Isozyme 3, Mitochondrial; GS1-358P8.4; Pyruvate Dehyd
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AA Sequence
RLFRWLLKQPVPKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLLNRPSVGLVQSWYMQSFLELLEYENKSPEDPQVLDNFLQVLIKVRNRHNDVVPTMAQGVIEYKEKFGFDPFISTNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKALSSESFERLPVFNKSAWRHYKTTPEADDWSNPSSEPRDASKYKAKQ
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Predicted Molecular Mass
74.5 kDa
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Molecular Weight
Approximately 74 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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