PGK2 Protein, Human (sf9, His)

The PGK2 protein is indispensable for sperm motility and male fertility, and studies have confirmed the critical role of the PGK2 protein in these processes. Its presence is essential to ensure the normal movement and function of sperm. PGK2 Protein, Human (sf9, His) is the recombinant human-derived PGK2 protein, expressed by Sf9 insect cells , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

The PGK2 protein is indispensable for sperm motility and male fertility, and studies have confirmed the critical role of the PGK2 protein in these processes. Its presence is essential to ensure the normal movement and function of sperm. PGK2 Protein, Human (sf9, His) is the recombinant human-derived PGK2 protein, expressed by Sf9 insect cells , with N-His labeled tag.

Background

The PGK2 protein is a vital component in the context of male reproductive biology, as it plays an essential role in sperm motility and contributes significantly to male fertility. Although it is indispensable for sperm movement and functionality, PGK2 is not deemed necessary for the completion of spermatogenesis, as suggested by similarities observed in related processes. This dual functionality underscores the specialized role of PGK2 in facilitating the dynamic and intricate processes involved in sperm motility, highlighting its critical importance in male reproductive success while not being a requisite factor for the earlier stages of spermatogenesis.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • His
    • PGK2 (S2-M417)
      Accession # P07205
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    PGK2; Phosphoglycerate Kinase Autosomal Pseudogene; Phosphoglycerate Kinase 2; Epididymis Secretory Protein Li 272; Phosphoglycerate Kinase, Testis Specific; Testicular Tissue Protein Li 139; PGKPS; Phosphoglycerate Kinase; PGK-2; DJ417L20.2; PGKB; HEL-S-

  • AA Sequence

    SLSKKLTLDKLDVRGKRVIMRVDFNVPMKKNQITNNQRIKASIPSIKYCLDNGAKAVVLMSHLGRPDGVPMPDKYSLAPVAVELKSLLGKDVLFLKDCVGAEVEKACANPAPGSVILLENLRFHVEEEGKGQDPSGKKIKAEPDKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPHKASGFLMKKELDYFAKALENPVRPFLAILGGAKVADKIQLIKNMLDKVNEMIIGGGMAYTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKNGVRITFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLIVWNGPLGVFEWDAFAKGTKALMDEIVKATSKGCITVIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKILPGVEALSNM

  • Predicted Molecular Mass

    46.9 kDa

  • Molecular Weight

    Approximately 44 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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