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PGLYRP4/PGRP-I beta Protein, Human (HEK293, His)

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Based on 1 Customer Validation

PGLYRP4/PGRP-I beta protein is a model receptor that binds to the cell wall peptide peptidoglycan (PGN) in Gram-positive bacteria and exerts bactericidal activity by interfering with peptidoglycan biosynthesis. Its antimicrobial properties extend to Gram-negative bacteria, although it also binds to them. PGLYRP4/PGRP-I beta Protein, Human (HEK293, His) is the recombinant human-derived PGLYRP4/PGRP-I beta protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for PGLYRP4/PGRP-I beta Protein, Human (HEK293, His)
  • Experimental Validation Results for PGLYRP4/PGRP-I beta Protein, Human (HEK293, His)
  • PGLYRP4/PGRP-I beta Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

PGLYRP4/PGRP-I beta protein is a model receptor that binds to the cell wall peptide peptidoglycan (PGN) in Gram-positive bacteria and exerts bactericidal activity by interfering with peptidoglycan biosynthesis. Its antimicrobial properties extend to Gram-negative bacteria, although it also binds to them. PGLYRP4/PGRP-I beta Protein, Human (HEK293, His) is the recombinant human-derived PGLYRP4/PGRP-I beta protein, expressed by HEK293 , with C-His labeled tag.

Background

PGLYRP4/PGRP-I beta Protein, a pattern receptor, exhibits binding affinity to murein peptidoglycans (PGN) found in Gram-positive bacteria, showcasing bactericidal activity against this bacterial group. Its antimicrobial properties may be attributed to its interference with peptidoglycan biosynthesis, leading to the killing of Gram-positive bacteria. Additionally, PGLYRP4/PGRP-I beta demonstrates bacteriostatic activity against Gram-negative bacteria, despite also binding to this bacterial class. As part of innate immunity, this protein plays a crucial role in recognizing and responding to bacterial pathogens. It forms homodimers connected by disulfide bonds and can heterodimerize with PGLYRP3, further expanding its functional repertoire in host defense mechanisms.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized peptidoglycan at 1 μg/mL (100 μL/well) on the plate can bind Biotinylated Human PGLYRP4/PGRP-I beta Protein. The ED50 for this effect is 3.541 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized peptidoglycan at 1 μg/mL (100 μL/well) on the plate can bind Biotinylated Human PGLYRP4/PGRP-I beta Protein. The ED50 for this effect is 3.541 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q96LB8-1 (D18-H373)

  • Gene ID
    57115  [NCBI]
  • Molecular Construction
    • N-term
    • PGLYRP4 (D18-H373)
      Accession # Q96LB8-1
    • His
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    PGLYRP4; SBBI67; Peptidoglycan Recognition Protein 4; Peptidoglycan Recognition Protein Intermediate Beta; PGLYRPIbeta; Peptidoglycan Recognition Protein I-Beta; PGRPIB; PGRP-I-Beta; PGRP-Ibeta; Peptidoglycan Recognition Protein I Beta Precursor

  • AA Sequence

    DSSWNKTQAKQVSEGLQYLFENISQLTEKGLPTDVSTTVSRKAWGAEAVGCSIQLTTPVNVLVIHHVPGLECHDQTVCSQRLRELQAHHVHNNSGCDVAYNFLVGDDGRVYEGVGWNIQGVHTQGYNNISLGFAFFGTKKGHSPSPAALSAMENLITYAVQKGHLSSSYVQPLLGKGENCLAPRQKTSLKKACPGVVPRSVWGARETHCPRMTLPAKYGIIIHTAGRTCNISDECRLLVRDIQSFYIDRLKSCDIGYNFLVGQDGAIYEGVGWNVQGSSTPGYDDIALGITFMGTFTGIPPNAAALEAAQDLIQCAMVKGYLTPNYLLVGHSDVARTLSPGQALYNIISTWPHFKH

  • Molecular Weight

    Approximately 50-72 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, pH 8.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized peptidoglycan at 1 μg/mL (100 μL/well) on the plate can bind Biotinylated Human PGLYRP4/PGRP-I beta Protein. The ED50 for this effect is 3.541 μg/mL.

  • PGLYRP4/PGRP-I beta Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for PGLYRP4/PGRP-I beta Protein, Human (HEK293, His)
  • Experimental Validation Results for PGLYRP4/PGRP-I beta Protein, Human (HEK293, His)
  • PGLYRP4/PGRP-I beta Protein, Human (HEK293, His) Product Image