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PRKCI Protein, Human (sf9, His)

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PKC iota proteins are members of the calcium-dependent cell adhesion protein family and play critical roles in cellular interactions. PKC iota acts through a calcium-dependent mechanism and exhibits a preference for homogeneous interacting, connecting cells. PRKCI Protein, Human (sf9, His) is the recombinant human-derived PKC iota, expressed by Sf9 insect cells, with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

PKC iota proteins are members of the calcium-dependent cell adhesion protein family and play critical roles in cellular interactions. PKC iota acts through a calcium-dependent mechanism and exhibits a preference for homogeneous interacting, connecting cells. PRKCI Protein, Human (sf9, His) is the recombinant human-derived PKC iota, expressed by Sf9 insect cells, with C-His labeled tag.

Background

Cadherin-17, belonging to the family of calcium-dependent cell adhesion proteins, functions as a pivotal component in cellular interactions. Operating through a calcium-dependent mechanism, cadherins like CDH17 exhibit a propensity for homophilic interactions, preferentially engaging with themselves in connecting cells. This unique property positions cadherins to potentially contribute to the intricate sorting processes of heterogeneous cell types. Specifically, LI-cadherin, a subtype within this family, may play a crucial role in the morphological organization of vital organs such as the liver and intestine. This highlights the diverse and specialized functions that cadherins, including CDH17, fulfill in maintaining tissue integrity and structural organization within specific physiological contexts.

Verified Bioactivity

The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-8*His
  • Accession

    P41743 (M10-V596)

  • Gene ID
    5584
  • Protein Length

    Partial

  • Synonyms

    PRKCI; PKCI; Prev. DXS1179E; PRKC-Lambda/Iota; Protein Kinase C Iota Type; APKC-Lambda/Iota; NPKC-Iota; Protein Kinase C, Iota; Protein Kinase C Iota; Atypical Protein Kinase C-Lambda/Iota

  • AA Sequence

    MSHTVAGGGSGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCNEVRDMCSFDNEQLFTMKWIDEEGDPCTVSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTIECGRHSLPQEPVMPMDQSSMHSDHAQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAASVLKSFLNKDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV

  • Molecular Weight

    Approximately 90-110 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM GSH, 0.5 mM PMSF, 10% glycerol, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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