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PKN1 Protein, Human (sf9, His, Flag)

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PKN1 Protein, Human (sf9, His, Flag) is the recombinant human-derived PKN1, expressed by Sf9 insect cells , with His, Flag labeled tag. ,

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • PKN1 Protein, Human (sf9, His, Flag) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

PKN1 Protein, Human (sf9, His, Flag) is the recombinant human-derived PKN1, expressed by Sf9 insect cells , with His, Flag labeled tag. ,

Background

PKN1 is a PKC-related serine/threonine-protein kinase involved in diverse biological processes, including regulation of actin cytoskeletal intermediate filaments, cell migration, tumor cell invasion, and transcriptional regulation. It functions within a signaling cascade initiated by adrenergic receptor ADRA1B activation, leading to MAPK14 activation. PKN1 modulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL, and NEFM, inhibiting their polymerization. Additionally, it phosphorylates MAPT/Tau at Ser-575, Ser-637, and Ser-669, reducing its microtubule-binding affinity and disrupting tubulin assembly. PKN1 acts as a key coactivator of androgen receptor (AR)-dependent transcription by targeting AR-regulated genes and mediating phosphorylation of histone H3 at Thr-11 (H3T11ph), an epigenetic mark promoting KDM4C/JMJD2C-dependent demethylation of H3K9me. It also phosphorylates HDAC5, HDAC7, and HDAC9, impairing their nuclear import. Other substrates include PPP1R14A (Thr-38), MARCKS (Ser-159, Ser-163, Ser-170), GFAP, and in vitro, RPS6.

Verified Bioactivity

The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-His;N-Avi;N-Flag
  • Accession

    Q16512-1 (M1-C942)

  • Gene ID
    5585
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    PKN1; Protein Kinase PKN-Alpha; Prev. PRKCL1; PAK-1; PAK1; Non-Specific Serine/Threonine Protein Kinase; PRK1; Serine/Threonine Protein Kinase N; PKN; Protein Kinase C-Related Kinase 1; Protein Kinase C-Like 1; Protein-Kinase C-Related Kinase 1; DBK; Seri

  • AA Sequence

    MASDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC

  • Molecular Weight

    Approximately 120 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 80%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 20% glycerol, 1mMDTT.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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