PNO1 Protein, Human (His)
Based on 1 Customer Validation
PNO1 Protein, Human (His) is the recombinant human-derived PNO1, expressed by E. coli , with C-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
PNO1 Protein, Human (His) is the recombinant human-derived PNO1, expressed by E. coli , with C-6*His labeled tag.
PNO1 is part of the small subunit (SSU) processome and serves as the first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, numerous ribosome biogenesis factors, an RNA chaperone, and ribosomal proteins associate with the nascent pre-rRNA, working together to facilitate RNA folding, modifications, rearrangements, and cleavage, as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Additionally, PNO1 positively regulates the dimethylation of two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA. by similar: 34516797, 25851604
Measured in a cell proliferation assay using HCT116 cells. The ED50 for this effect is typically 0.4094-0.5623 μg/mL.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
Q9NRX1 (M1-F252)
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Gene ID56902
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Protein Length
Full Length
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Synonyms
PNO1; RNA-Binding Protein PNO1; Prev. KHRBP1; Partner Of NOB1 Homolog (S. Cerevisiae); RRP20; KH-Type RNA Binding Protein 1; KH-Type RNA-Binding Protein 1; Partner Of NOB1; Partner Of NOB1 Homolog; RNA Binding Protein
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AA Sequence
MESEMETQSARAEEGFTQVTRKGGRRAKKRQAEQLSAAGEGGDAGRMDTEEARPAKRPVFPPLCGDGLLSGKEETRKIPVPANRYTPLKENWMKIFTPIVEHLGLQIRFNLKSRNVEIRTCKETKDVSALTKAADFVKAFILGFQVEDALALIRLDDLFLESFEITDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTRTRIVLADVKVHILGSFQNIKMARTAICNLILGNPPSKVYGNIRAVASRSADRF
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Predicted Molecular Mass
27.9 kDa
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Molecular Weight
Approximately 32 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, 8-10% trehalose, pH 8.0.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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