PNPLA3 Protein, Human (I148M, HEK293, His)
PNPLA3 Protein, Human (I148M, HEK293, His) is the recombinant human-derived PNPLA3 protein, expressed by HEK293, with C-6*His tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
PNPLA3 Protein, Human (I148M, HEK293, His) is the recombinant human-derived PNPLA3 protein, expressed by HEK293, with C-6*His tag.
Background
PNPLA3 specifically catalyzes the coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to produce phosphatidic acid (PA), a key metabolic intermediate and precursor for both triglycerides and glycerophospholipids. It does not esterify other lysophospholipids, utilizing long-chain (at least C16) fatty acyl-CoAs such as arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA, and to a lesser extent, palmitoyl-CoA as acyl donors. Additionally, PNPLA3 exhibits low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities, suggesting a potential role in triglyceride acyl-chain remodeling. In vitro studies indicate hydrolytic activity against triacylglycerol, diacylglycerol, and monoacylglycerol, with a strong preference for oleic acid as the acyl moiety. However, its triacylglycerol hydrolase activity is debated and may be minimal. PNPLA3 also possesses phospholipase A2 activity. by similar: The enzyme's activity varies across studies, particularly regarding the extent of its triacylglycerol hydrolase function.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q9NST1 (M1-L481, I148M)
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Gene ID80339
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Molecular Construction
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N-term
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(M1-L481)
Accession # Q9NST1 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
PNPLA3; Calcium-Independent Phospholipase A2-Epsilon; Prev. C22orf20; Acylglycerol Transacylase; Prev. ADPN; IPLA2-Epsilon; Adiponutrin; DJ796I17.1; Patatin Like Domain 3, 1-Acylglycerol-3-Phosphate O-Acyltransferase; FLJ22012; Patatin-Like Phospholipase
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AA Sequence
MYDAERGWSLSFAGCGFLGFYHVGATRCLSEHAPHLLRDARMLFGASAGALHCVGVLSGIPLEQTLQVLSDLVRKARSRNIGIFHPSFNLSKFLRQGLCKCLPANVHQLISGKIGISLTRVSDGENVLVSDFRSKDEVVDALVCSCFMPFYSGLIPPSFRGVRYVDGGVSDNVPFIDAKTTITVSPFYGEYDICPKVKSTNFLHVDITKLSLRLCTGNLYLLSRAFVPPDLKVLGEICLRGYLDAFRFLEEKGICNRPQPGLKSSSEGMDPEVAMPSWANMSLDSSPESAALAVRLEGDELLDHLRLSILPWDESILDTLSPRLATALSEEMKDKGGYMSKICNLLPIRIMSYVMLPCTLPVESAIAIVQRLVTWLPDMPDDVLWLQWVTSQVFTRVLMCLLPASRSQMPVSSQQASPCTPEQDWPCWTPCSPKGCPAETKAEATPRSILRSSLNFFLGNKVPAGAEGLSTFPSFSLEKSL
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Predicted Molecular Mass
55.2 kDa
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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