Podocin/NPHS2 Protein, Human (His, Myc)
Podocin/NPHS2 protein is encoded by the NPHS2 gene and controls glomerular permeability as an important linker between the plasma membrane and cytoskeleton. Interactions with phenylephrine/NPHS1, KIRREL1, and CD2AP emphasize their integral role in the regulation of podocyte function. Podocin/NPHS2 Protein, Human (His, Myc) is the recombinant human-derived Podocin/NPHS2 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Podocin/NPHS2 protein is encoded by the NPHS2 gene and controls glomerular permeability as an important linker between the plasma membrane and cytoskeleton. Interactions with phenylephrine/NPHS1, KIRREL1, and CD2AP emphasize their integral role in the regulation of podocyte function. Podocin/NPHS2 Protein, Human (His, Myc) is the recombinant human-derived Podocin/NPHS2 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.
Background
Podocin, encoded by the NPHS2 gene, is instrumental in governing glomerular permeability, likely serving as a pivotal linker between the plasma membrane and the cytoskeleton. Its interactions with nephrin/NPHS1, KIRREL1, and CD2AP underscore its integral role in the intricate regulation of podocyte function. Direct engagement with DDN, inferred by similarity, further highlights Podocin's participation in essential cellular processes. Collectively, these interactions emphasize Podocin's crucial contribution to maintaining the structural integrity and dynamic functionality of the glomerular filtration barrier.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-10*His;C-Myc
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Accession
Q9NP85-1 (C124-L383)
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Molecular Construction
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N-term
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10*His
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NPHS2 (C124-L383)
Accession # Q9NP85-1 -
Myc
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C-term
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Protein Length
Partial
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Synonyms
NPHS2; PDCN; NPHS2 Stomatin Family Member, Podocin; Nephrosis 2, Idiopathic, Steroid-Resistant (Podocin); Podocin; NPHS2, Podocin; SRN1; Podocin Splice Variant
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AA Sequence
CVKVVQEYERVIIFRLGHLLPGRAKGPGLFFFLPCLDTYHKVDLRLQTLEIPFHEIVTKDMFIMEIDAICYYRMENASLLLSSLAHVSKAVQFLVQTTMKRLLAHRSLTEILLERKSIAQDAKVALDSVTCIWGIKVERIEIKDVRLPAGLQHSLAVEAEAQRQAKVRMIAAEAEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTEKPSTVVLPLPFDLLNCLSSPSNRTQGSLPFPSPSKPVEPLNPKKKDSPML
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Predicted Molecular Mass
34.1 kDa
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 200 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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