1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. Betacellulin
  5. Betacellulin/BTC Protein, Mouse (HEK293)

Betacellulin/BTC Protein, Mouse (HEK293)

Cat. No.: HY-P7142
COA Handling Instructions

Probetacellulin Protein, Mouse (HEK293), a member of the epidermal growth factor (EGF) family, induces differentiation of pancreatic β-cells and promotes regeneration of β-cells in experimental diabetes.

For research use only. We do not sell to patients.

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50 μg $260 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

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Description

Probetacellulin Protein, Mouse (HEK293), a member of the epidermal growth factor (EGF) family, induces differentiation of pancreatic β-cells and promotes regeneration of β-cells in experimental diabetes.

Background

Betacellulin (BTC), a member of the epidermal growth factor (EGF) family, induces differentiation of pancreatic β-cells and promotes regeneration of β-cells in experimental diabetes. BTC stimulates DNA synthesis in fibroblasts and vascular smooth muscle cells. BTC plays a role in regulating growth and/or differentiation of endocrine precursor cells of the fetal pancreas. BTC is found to convert amylase-secreting pancreatic AR42J cells into insulin-producing cells and to have a mitogenic effect in human undifferentiated pancreatic epithelial cells[1].

Biological Activity

The ED50 is <0.08 ng/mL as measured by 3T3 cells.

Species

Mouse

Source

HEK293

Tag

Tag Free

Accession

Q05928 (D32-Y111)

Gene ID

12223  [NCBI]

Molecular Construction
N-term
BTC (D32-Y111)
Accession # Q05928
C-term
Synonyms
rMuBetacellulin; BTC; Probetacellulin
AA Sequence

DGNTTRTPETNGSLCGAPGENCTGTTPRQKVKTHFSRCPKQYKHYCIHGRCRFVVDEQTPSCICEKGYFGARCERVDLFY

Molecular Weight

19-24 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

Betacellulin/BTC Protein, Mouse (HEK293) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Betacellulin/BTC Protein, Mouse (HEK293)
Cat. No.:
HY-P7142
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