Prolyl Endopeptidase/PREP Protein, Mouse (sf9, His)
Based on 1 Customer Validation
The prolyl endopeptidase (PREP) protein is an enzyme capable of cleaving peptide bonds at the C-terminus of prolyl residues within peptides, particularly peptides up to about 30 amino acids in length.This enzymatic activity suggests a role for PREP in selective processing of peptides, particularly toward proline-containing substrates.Prolyl Endopeptidase/PREP Protein, Mouse (sf9, His) is the recombinant mouse-derived Prolyl Endopeptidase/PREP protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Mouse
- Source: Sf9 insect cells
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The prolyl endopeptidase (PREP) protein is an enzyme capable of cleaving peptide bonds at the C-terminus of prolyl residues within peptides, particularly peptides up to about 30 amino acids in length.This enzymatic activity suggests a role for PREP in selective processing of peptides, particularly toward proline-containing substrates.Prolyl Endopeptidase/PREP Protein, Mouse (sf9, His) is the recombinant mouse-derived Prolyl Endopeptidase/PREP protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
Prolyl Endopeptidase (PREP) is an enzyme that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides, particularly those of shorter lengths, up to approximately 30 amino acids. This specificity for prolyl residues highlights PREP's role in the selective hydrolysis of peptide bonds in specific substrates. The enzyme's activity suggests its involvement in the regulation of peptides containing proline, which can be significant in various cellular processes. Although further research is required to fully elucidate its physiological functions, the identified substrate specificity positions PREP as a key player in the processing and modulation of proline-containing peptides (
Verified Bioactivity
Measured by its ability to convert 50 μM substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC) that incubate in kinetic mode for 5 minutes. The specific activity is 21105.10 pmol/min/µg.
Technical Parameters
-
Species Mouse
-
Source Sf9 insect cells
-
Tag N-His
-
Accession
Q9QUR6 (L2-Q710)
-
Molecular Construction
-
N-term
-
His
-
PREP (L2-Q710)
Accession # Q9QUR6 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
PREP; PEP; Prolyl Endopeptidase; PE; Post-Proline Cleaving Enzyme; DJ355L5.1 (Prolyl Endopeptidase); Prolyl Oligopeptidase
-
AA Sequence
LSFQYPDVYRDETSVQEYHGHKICDPYSWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLYKERMTELYDYPKYSCHFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVFLDPNTLSDDGTVALRGYAFSEDGEYFAYGLSASGSDWVTIKFMKVDGAKELPDVLERVKFTCMAWTHDGKGMFYNSYPQQDGKSDGTETSTNLHQKLCYHVLGTDQSEDILCAEFPDEPKWMGGAELSDDGRYVLLSIWEGCDPVNRLWYCDLQQEPNGITGILKWVKLIDNFEGEYDYVTNEGTVFTFKTNRNSPNYRLINIDFTDPDESKWKVLVPEHEKDVLEWVACVRSNFLVLCYLHDVKNILQLHDLTTGALLKTFPLDVGSVVGYSGRKKDSEIFYQFTSFLSPGVIYHCDLTKEELEPMVFREVTVKGIDAADYQTIQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGVLAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKFTIGHAWTTDYGCSDTKQHFEWLLKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNIEWIQ
-
Predicted Molecular Mass
82.9 kDa
-
Molecular Weight
Approximately 79-83 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% Glycerol, 3 mM DTT, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (240 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
