PRSS2/Trypsin-2 Protein, Mouse (HEK293, His)

PRSS2 Protein plays an important role in the metastasis and recurrence of multiple tumor types through its actions of destroying the ECM and activating MMPs. PRSS2/Trypsin-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived PRSS2/Trypsin-2 protein, expressed by HEK293 , with C-His labeled tag.

PRSS2 Protein plays an important role in the metastasis and recurrence of multiple tumor types through its actions of destroying the ECM and activating MMPs. PRSS2 enables calcium ion binding activity and serine-type endopeptidase activity. PRSS2 is involved in proteolysis, response to caffeine and response to nicotine.PRSS2 acst upstream of or within digestion^[1][2][3].

Measured by its ability to cleave the fluorogenic peptide substrate, Mca- RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >1500 pmoles/min/μg.

Mouse

HEK293

C-His

Q792Y6 (F16-N246)

22072  [NCBI]

Full Length of Mature Protein

FPVDDDDKIVGGYTCRESSVPYQVSLNAGYHFCGGSLINDQWVVSAAHCYKYRIQVRLGEHNINVLEGNEQFVDSAKIIRHPNYNSWTLDNDIMLIKLASPVTLNARVASVPLPSSCAPAGTQCLISGWGNTLSNGVNNPDLLQCVDAPVLPQADCEASYPGDITNNMICVGFLEGGKDSCQGDSGGPVVCNGELQGIVSWGYGCAQPDAPGVYTKVCNYVDWIQNTIADN

Approximately 32 kDa,based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 22.5% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.