PSME2 Protein, Human (His)

PSME2 Protein, vital for immunoproteasome assembly, is crucial for efficient antigen processing. In collaboration with PSME1, the PA28 activator complex modifies the proteasome's cleavage pattern, actively contributing to class I binding peptide generation. The hexameric ring structure of this heterodimeric complex underscores its significance in orchestrating processes essential for immune responses and antigen presentation. PSME2 Protein, Human (His) is the recombinant human-derived PSME2 protein, expressed by E. coli , with N-His labeled tag.

PSME2 Protein plays a crucial role in immunoproteasome assembly and is essential for efficient antigen processing. The PA28 activator complex, composed of PSME1 and PSME2 heterodimer, actively contributes to the generation of class I binding peptides by modifying the cleavage pattern of the proteasome. This heterodimeric complex forms a hexameric ring structure, highlighting its significance in orchestrating processes vital for immune responses and antigen presentation.

1.Immobilized Mouse PSME2 at 2 μg/mL (100 μL/well) can bind Anti- PSME2 Antibody, The ED₅₀ for this effect is 26.49 ng/mL.
2.Measured by its ability to activate the h20S Proteasome to cleave the substrate suc-LLVY-AMC. The specific activity is 2291.82 pmol/min/μg.

Human

E. coli

N-His

Q9UL46 (M1-Y239)

5721  [NCBI]

Full Length

MAKPCGVRLSGEARKQVEVFRQNLFQEAEEFLYRFLPQKIIYLNQLLQEDSLNVADLTSLRAPLDIPIPDPPPKDDEMETDKQEKKEVHKCGFLPGNEKVLSLLALVKPEVWTLKEKCILVITWIQHLIPKIEDGNDFGVAIQEKVLERVNAVKTKVEAFQTTISKYFSERGDAVAKASKETHVMDYRALVHERDEAAYGELRAMVLDLRAFYAELYHIISSNLEKIVNPKGEEKPSMY

Approximately 29 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.0, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.