2. Protéines recombinantes
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. pulB Protein, Pullulanibacillus naganoensis (FLAG, His)

pulB Protein, Pullulanibacillus naganoensis (FLAG, His)

Cat. No.: HY-P702160
Instruction de manipulation Technical Support

PulB is a protein belonging to the glycosyl hydrolase 13 family. The glycosyl hydrolase 13 family contains enzymes involved in the breakdown of complex carbohydrates by catalyzing the hydrolysis of glycosidic bonds. pulB Protein, Pullulanibacillus naganoensis (FLAG, His) is the recombinant pulB protein, expressed by E. coli , with N-6*His, N-Flag labeled tag.

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  • Description

Description

PulB is a protein belonging to the glycosyl hydrolase 13 family. The glycosyl hydrolase 13 family contains enzymes involved in the breakdown of complex carbohydrates by catalyzing the hydrolysis of glycosidic bonds. pulB Protein, Pullulanibacillus naganoensis (FLAG, His) is the recombinant pulB protein, expressed by E. coli , with N-6*His, N-Flag labeled tag.
Background

PulB is a protein that belongs to the glycosyl hydrolase 13 family. The glycosyl hydrolase 13 family comprises enzymes involved in the breakdown of complex carbohydrates by catalyzing the hydrolysis of glycosidic bonds. While specific information about PulB is limited, its membership in this family suggests that it may have enzymatic activity related to carbohydrate metabolism. The exact function and role of PulB are not well characterized, and further research is needed to fully understand its specific enzymatic activity, substrate specificity, and potential implications in biological processes.
Species

Others
Source

E. coli
Tag

N-6*His;N-Flag
Accession

G9JLV4 (D1-K926)
Gene ID

/
Molecular Construction
N-term
Flag-6*His
pulB (D1-K926)
Accession # G9JLV4
C-term
Protein Length

Full Length
Synonyms
pulB; Alpha-dextrin endo-1; 6-alpha-glucosidase; Pullulan 6-glucanohydrolase
AA Sequence

DGNTTNIVVHYFRPSGDYTDWNLWMWPENGDGAEYDFNQPTDSYGEVASVDIPGNPSQVGIIVRKGNWDAKDIDSDRYIDLSKGHEIWLVQGNSQIFYSEKDAEAAAQPAVSNAYLDASNQVLVKLSQPFTLGEGSSGFTVHDDTANKDIPVTSVSDANQVTAVLAGTFQHIFGGSDWAPDNHNTLLKKVNSNLYQFSGNLPEGNYQYKVALNDSWNNPSYPSDNINLTVPAGGAHVTFSYIPSTHAVYDTINNPNADLQVDSSGVKTDLVAVTLGENPDVSHTLSIQTEDYQAGQVIPRKVLDSSQYYYSGDDLGNTYTKNATTFKVWAPTSTQVNVLLYNSATGAVTKTVPMTASGHGVWEATVNQDLENWYYMYEVTGQGSTRTAVDPYATAIAPNGTRGMIVDLAKTDPAGWESDKHITPKNIEDEVIYEMDVRDFSIDSNSGMKNKGKYLALTEKGTKGPDNVKTGVDSLKQLGITHVQLQPVFAFNSVNENDPTQYNWGYDPRNYNVPEGQYATNANGTTRIKEFKEMVLSLHQDHIGVNMDVVYNHTFATQISDFDKIVPEYYYRTDDAGNYTNGSGTGNEIAAERPMVQKFIIDSLKFWVNEYHVDGFRFDLMALLGKDTMSKAATQLHAIDPGIALYGEPWTGGTSALPADQLLTKGAQKGMGVAVFNDNLRNGLDGSVFDSSAQGFATGATGLTDAIKNGVEGSINDFTASPGETINYVTSHDNYTLWDKIAQSNPNDSEADRIKMDELAQAIVMTSQGIPFMQGGEEMLRTKGGNDNSYNAGDVVNEFDWSRKAQYPDVFNYYSGLIHLRLDHPAFRMTTANEINSHLQFLNSPENTVAYELSDHANKDTWGNIVVIYNPNKTAETINLPSGKWEINATSGKVGESTLGQAEGSVQVPGISMMILHQEVSPSDGK

Pureté

≥ 90%, as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Instruction de manipulation (2659 KB)

pulB Protein, Pullulanibacillus naganoensis (FLAG, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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