PYCARD Protein, Human (HEK293, His-Myc)

PYCARD proteins are key mediators of apoptosis and inflammation and promote caspase-mediated apoptosis, specifically involving caspase-8 and, in certain cells, caspase-9. It activates the mitochondrial apoptotic pathway, promoting caspase-8-dependent BID maturation and BAX-dependent apoptosis through caspase-9, -2, and -3. PYCARD Protein, Human (HEK293, His-Myc) is the recombinant human-derived PYCARD protein, expressed by HEK293 , with C-Myc, N-10*His labeled tag. The total length of PYCARD Protein, Human (HEK293, His-Myc) is 195 a.a., with molecular weight of ~30 kDa.

PYCARD protein serves as a pivotal mediator in apoptosis and inflammation, playing multifaceted roles in cellular processes. It facilitates caspase-mediated apoptosis, predominantly involving caspase-8 and, in specific cell types, caspase-9. PYCARD is integral to the activation of the mitochondrial apoptotic pathway, promoting caspase-8-dependent proteolytic maturation of BID independently of FADD, as well as mediating mitochondrial translocation of BAX, initiating BAX-dependent apoptosis coupled with the activation of caspase-9, -2, and -3. Furthermore, PYCARD is a crucial adapter in the assembly of various inflammasomes, including NLRP1, NLRP2, NLRP3, NLRP6, AIM2, and likely IFI16, leading to caspase-1 activation, pro-inflammatory cytokine processing, and inflammation. In cooperation with NOD2, PYCARD participates in an inflammasome activated by bacterial muramyl dipeptide. Additionally, it may be involved in RIGI-triggered pro-inflammatory responses and inflammasome activation. In adaptive immunity, PYCARD contributes to dendritic cell maturation, stimulating T-cell immunity, and modulates cytoskeletal rearrangements associated with chemotaxis and antigen uptake. Furthermore, it plays a role in the transcriptional activation of cytokines and chemokines, independently of the inflammasome, through signaling pathways involving AP-1, NF-kappa-B, MAPK, and caspase-8. The regulatory functions of PYCARD include modulation of NF-kappa-B induction at the IKK complex level, inhibiting kinase activity of CHUK and IKBK. It is proposed to compete with RIPK2, down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation, and activating interleukin-1 beta processing. In host resistance to DNA virus infection, PYCARD likely induces the cleavage and inactivation of CGAS in the presence of cytoplasmic double-stranded DNA, showcasing its diverse and critical roles in cellular responses.

Human

HEK293

C-Myc;N-10*His

Q9ULZ3 (M1-S195)

29108

MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS

Approximately 30 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.