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PYCARD Protein, Human (HEK293, His-Myc)

Cat. No.: HY-P701308
COA Handling Instructions

PYCARD proteins are key mediators of apoptosis and inflammation and promote caspase-mediated apoptosis, specifically involving caspase-8 and, in certain cells, caspase-9. It activates the mitochondrial apoptotic pathway, promoting caspase-8-dependent BID maturation and BAX-dependent apoptosis through caspase-9, -2, and -3. PYCARD Protein, Human (HEK293, His-Myc) is the recombinant human-derived PYCARD protein, expressed by HEK293 , with C-Myc, N-10*His labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $420 In-stock
50 μg $800 In-stock
100 μg $1280 In-stock
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Description

PYCARD proteins are key mediators of apoptosis and inflammation and promote caspase-mediated apoptosis, specifically involving caspase-8 and, in certain cells, caspase-9. It activates the mitochondrial apoptotic pathway, promoting caspase-8-dependent BID maturation and BAX-dependent apoptosis through caspase-9, -2, and -3. PYCARD Protein, Human (HEK293, His-Myc) is the recombinant human-derived PYCARD protein, expressed by HEK293 , with C-Myc, N-10*His labeled tag.

Background

PYCARD protein serves as a pivotal mediator in apoptosis and inflammation, playing multifaceted roles in cellular processes. It facilitates caspase-mediated apoptosis, predominantly involving caspase-8 and, in specific cell types, caspase-9. PYCARD is integral to the activation of the mitochondrial apoptotic pathway, promoting caspase-8-dependent proteolytic maturation of BID independently of FADD, as well as mediating mitochondrial translocation of BAX, initiating BAX-dependent apoptosis coupled with the activation of caspase-9, -2, and -3. Furthermore, PYCARD is a crucial adapter in the assembly of various inflammasomes, including NLRP1, NLRP2, NLRP3, NLRP6, AIM2, and likely IFI16, leading to caspase-1 activation, pro-inflammatory cytokine processing, and inflammation. In cooperation with NOD2, PYCARD participates in an inflammasome activated by bacterial muramyl dipeptide. Additionally, it may be involved in RIGI-triggered pro-inflammatory responses and inflammasome activation. In adaptive immunity, PYCARD contributes to dendritic cell maturation, stimulating T-cell immunity, and modulates cytoskeletal rearrangements associated with chemotaxis and antigen uptake. Furthermore, it plays a role in the transcriptional activation of cytokines and chemokines, independently of the inflammasome, through signaling pathways involving AP-1, NF-kappa-B, MAPK, and caspase-8. The regulatory functions of PYCARD include modulation of NF-kappa-B induction at the IKK complex level, inhibiting kinase activity of CHUK and IKBK. It is proposed to compete with RIPK2, down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation, and activating interleukin-1 beta processing. In host resistance to DNA virus infection, PYCARD likely induces the cleavage and inactivation of CGAS in the presence of cytoplasmic double-stranded DNA, showcasing its diverse and critical roles in cellular responses.

Species

Human

Source

HEK293

Tag

C-Myc;N-10*His

Accession

Q9ULZ3-1 (M1-S195)

Gene ID

29108

Molecular Construction
N-term
10*His
PYCARD (M1-S195)
Accession # Q9ULZ3-1
C-term
Synonyms
PYCARD; PYD And CARD Domain Containing; CARD5; ASC; Apoptosis-Associated Speck-Like Protein Containing A CARD; TMS-1; Caspase Recruitment Domain-Containing Protein 5; TMS1; PYD And CARD Domain-Containing Protein; TMS; HASC
AA Sequence

MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS

Molecular Weight

Approximately 30 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PYCARD Protein, Human (HEK293, His-Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PYCARD Protein, Human (HEK293, His-Myc)
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