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Ribonuclease R (rnr) protein, E.coli (His)

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Based on 2 publication(s) in Google Scholar

Ribonuclease R (RNase R) is a 3'-5' exoribonuclease crucial for structured RNA maturation, degrading rRNAs, tRNAs, and SsrA/tmRNA. It modulates ompA mRNA stability in the stationary phase, exhibiting processive activity and helicase function. RNase R, along with PNPase, ensures rRNA quality during steady-state growth and contributes to virulence gene expression in E. coli. Ribonuclease R (rnr) protein, E.coli (His) is the recombinant E. coli-derived Ribonuclease R, expressed by E. coli , with N-6*His labeled tag.

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  • Species: E.coli
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Ribonuclease R (rnr) protein, E.coli (His)
  • Ribonuclease R (rnr) protein, E.coli (His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

Ribonuclease R (RNase R) is a 3'-5' exoribonuclease crucial for structured RNA maturation, degrading rRNAs, tRNAs, and SsrA/tmRNA. It modulates ompA mRNA stability in the stationary phase, exhibiting processive activity and helicase function. RNase R, along with PNPase, ensures rRNA quality during steady-state growth and contributes to virulence gene expression in E. coli. Ribonuclease R (rnr) protein, E.coli (His) is the recombinant E. coli-derived Ribonuclease R, expressed by E. coli , with N-6*His labeled tag.

Background

Ribonuclease R (RNase R) is a 3'-5' exoribonuclease that plays a crucial role in the maturation of structured RNAs, including rRNAs, tRNAs, and SsrA/tmRNA. It acts by releasing 5'-nucleoside monophosphates during the degradation process. Additionally, in the stationary phase, RNase R is involved in post-transcriptional regulation, specifically in the modulation of ompA mRNA stability. It exhibits processive activity, shortening RNA molecules to di- and trinucleotides. Interestingly, RNase R also possesses helicase activity, which is distinct from its RNase function. During starvation, RNases 2 and R (rnb and RNase R) contribute to the degradation of rRNA. However, under normal growth conditions, RNase R, along with PNPase, plays a more significant role in quality control of rRNA. These enzymes work together to ensure proper functioning and integrity of rRNA during steady-state growth. Additionally, RNase R is required for the expression of virulence genes in enteroinvasive strains of E. coli.

Verified Bioactivity

Measured by its ability to digest RNA, it reacted at 37°C for 30 minutes under a certain reaction system, and was identified by agar-gel electrophoresis. The optimal reaction concentration measured under the above conditions was 80 μg/mL-320 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species E.coli
  • Source E. coli
  • Tag N-6*His
  • Accession

    P21499 (M1-E813)

  • Gene ID
    948692
  • Molecular Construction
    • N-term
    • 6*His
    • RNase R (M1-E813)
      Accession # P21499
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    NR2E3; Rd7; Nuclear Receptor Subfamily 2 Group E Member 3; RNR; PNR; Nuclear Receptor Subfamily 2, Group E, Member 3; Photoreceptor-Specific Nuclear Receptor; NR2E3 Protein; Retina-Specific Nuclear Receptor; ESCS1; RP37; ESCS

  • AA Sequence

    MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVELHIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVEGRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEAGVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNMGTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDARDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPSTPLDREARNRGTSVYFPSQVIPMLPEVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQGDQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQTQRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLELPGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHFTSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRADEATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYYRFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTAREKAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIAAATKAKRAAKKKVAE

  • Predicted Molecular Mass

    92.1 kDa

  • Molecular Weight

    Approximately 88-100 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% TX-100, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Ribonuclease R (rnr) protein, E.coli (His) Product Image
    1/16
  • Experimental Validation Results for Ribonuclease R (rnr) protein, E.coli (His)
  • Ribonuclease R (rnr) protein, E.coli (His) Product Image