ROR1 Protein, Human (sf9, His)

ROR1 protein's minimal kinase activity in vitro suggests an improbable role as a tyrosine kinase in vivo. It serves as a receptor for WNT5A, activating NFkB signaling and potentially inhibiting WNT3A signaling. Notably, ROR1 plays a vital role in the inner ear, facilitating innervation of auditory hair cells by spiral ganglion neurons. ROR1 Protein, Human (sf9, His) is the recombinant human-derived ROR1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of ROR1 Protein, Human (sf9, His) is 331 a.a., with molecular weight of 55-70 kDa.

ROR1 protein exhibits very low kinase activity in vitro, suggesting an unlikely role as a tyrosine kinase in vivo. It functions as a receptor for the ligand WNT5A, activating downstream NFkB signaling pathways and potentially inhibiting WNT3A-mediated signaling. Notably, in the inner ear, ROR1 is crucial for facilitating the innervation of auditory hair cells by spiral ganglion neurons.

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Anti-ROR1 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human ROR1, His Tag with an affinity constant of 26.40 nM as determined in SPR assay (Biacore T200).

Human

Sf9 insect cells

C-His

Q01973 (M453-N783)

4919  [NCBI]

55-70 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 3 mM DTT, 10% glycerol, pH 7.4

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.