SARM1 Protein, Human (HEK293, His, HSA)
SARM1 Protein, Human (HEK293, His, HSA) is the recombinant human-derived SARM1, expressed by HEK293 , with N-Flag labeled tag. ,
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
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Biological Activity
Description
SARM1 Protein, Human (HEK293, His, HSA) is the recombinant human-derived SARM1, expressed by HEK293 , with N-Flag labeled tag. ,
Background
SARM1 is an NAD(+) hydrolase that plays a critical role in axonal degeneration following injury by regulating NAD(+) metabolism. It acts as a negative regulator of the MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death involving degeneration of the axon distal to the injury site. Wallerian degeneration is triggered by NAD(+) depletion: upon injury, SARM1 is activated and cleaves NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR), and nicotinamide, leading to cytoskeletal degradation and axon destruction. SARM1 can also hydrolyze NADP(+) but not other NAD(+)-related molecules. Additionally, it can activate neuronal cell death in response to stress. It regulates dendritic arborization via the MAPK4-JNK pathway (by similarity). Involved in innate immune responses, SARM1 inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-HSA;C-6*His
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Accession
Q6SZW1-1 (L28-T724)
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Gene ID23098
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Molecular Construction
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N-term
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SARM1 (L28-T724)
Accession # Q6SZW1-1 -
6*His-HSA
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
SARM1; Sterile Alpha And Armadillo Repeat Protein; Sterile Alpha And TIR Motif Containing 1; SAM Domain-Containing Protein 2; SAMD2; Tir-1 Homolog; SARM; NADase SARM1; Sterile Alpha And TIR Motif-Containing Protein 1; MyD88-5; NADP(+) Hydrolase SARM1; HSA
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AA Sequence
LAVPGPDGGGGTGPWWAAGGRGPREVSPGAGTEVQDALERALPELQQALSALKQAGGARAVGAGLAEVFQLVEEAWLLPAVGREVAQGLCDAIRLDGGLDLLLRLLQAPELETRVQAARLLEQILVAENRDRVARIGLGVILNLAKEREPVELARSVAGILEHMFKHSEETCQRLVAAGGLDAVLYWCRRTDPALLRHCALALGNCALHGGQAVQRRMVEKRAAEWLFPLAFSKEDELLRLHACLAVAVLATNKEVEREVERSGTLALVEPLVASLDPGRFARCLVDASDTSQGRGPDDLQRLVPLLDSNRLEAQCIGAFYLCAEAAIKSLQGKTKVFSDIGAIQSLKRLVSYSTNGTKSALAKRALRLLGEEVPRPILPSVPSWKEAEVQTWLQQIGFSKYCESFREQQVDGDLLLRLTEEELQTDLGMKSGITRKRFFRELTELKTFANYSTCDRSNLADWLGSLDPRFRQYTYGLVSCGLDRSLLHRVSEQQLLEDCGIHLGVHRARILTAAREMLHSPLPCTGGKPSGDTPDVFISYRRNSGSQLASLLKVHLQLHGFSVFIDVEKLEAGKFEDKLIQSVMGARNFVLVLSPGALDKCMQDHDCKDWVHKEIVTALSCGKNIVPIIDGFEWPEPQVLPEDMQAVLTFNGIKWSHEYQEATIEKIIRFLQGRSSRDSSAGSDTSLEGAAPMGPT
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Predicted Molecular Mass
144.5 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O . For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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