SARS-CoV Plpro/papain-like protease Protein (His)
Based on 1 Customer Validation
SARS CoV ORF1ab polyprotein (PP1ab) is a 7073 residues long polyprotein which further produces 5 non-structural proteins (NSPs) after processing through virally encoded proteases (NSP12-NSP16).All these 5 proteins are mainly involved in viral replication, assembly, and immune response modulation.SARS-CoV Plpro/papain-like protease Protein (His) is the recombinant Virus-derived SARS-CoV Plpro/papain-like protease protein, expressed by E.coli , with C-His labeled tag.
- Species: Virus
- Source: E. coli
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
SARS CoV ORF1ab polyprotein (PP1ab) is a 7073 residues long polyprotein which further produces 5 non-structural proteins (NSPs) after processing through virally encoded proteases (NSP12-NSP16).All these 5 proteins are mainly involved in viral replication, assembly, and immune response modulation.SARS-CoV Plpro/papain-like protease Protein (His) is the recombinant Virus-derived SARS-CoV Plpro/papain-like protease protein, expressed by E.coli , with C-His labeled tag.
Background
SARS CoV ORF1ab polyprotein (PP1ab) is a 7073 residues long polyprotein which further produces 5 non-structural proteins (NSPs) after processing through virally encoded proteases (NSP12-NSP16). All these 5 proteins are mainly involved in viral replication, assembly, and immune response modulation. Polyprotein degradation is mediated by main protease (Mpro) and papain-like protease (PLpro), PP1ab is thus processed into the individual NSPs that form the viral replication and transcription complex, The function of viral RNA transcription and replication is performed by a specific RNA dependent RNA polymerase (RdRp) region present in PP1ab [1][2].
MCE Validation Data
-
Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
-
Species Virus
-
Source E. coli
-
Tag C-His
-
Accession
AAX16193 (E1541-Y1859)
-
Gene ID/
-
Molecular Construction
-
N-term
-
SARS-CoV Plpro (E1541-Y1859)
Accession # AAX16193 -
His
-
C-term
-
-
Protein Length
Partial
-
Synonyms
orf1ab polyprotein
-
AA Sequence
EVKTIKVFTTVDNTNLHTQLVDMSMTYGQQFGPTYLDGADVTKIKPHVNHEGKTFFVLPSDDTLRSEAFEYYHTLDESFLGRYMSALNHTKKWKFPQVGGLTSIKWADNNCYLSSVLLALQQLEVKFNAPALQEAYYRARAGDAANFCALILAYSNKTVGELGDVRETMTHLLQHANLESAKRVLNVVCKHCGQKTTTLTGVEAVMYMGTLSYDNLKTGVSIPCVCGRDATQYLVQQESSFVMMSAPPAEYKLQQGTFLCANEYTGNYQCGHYTHITAKETLYRIDGAHLTKMLEYKGPVTDVFYKETSYTTTIKPVSY
-
Predicted Molecular Mass
36.9 kDa
-
Molecular Weight
Approximately 37 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as 0.22 μm filtered solution in 50 mM Tris, 0.1% Tween, 150 mM NaCl, pH 9.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
-
Data Sheet (236 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
