SARS-CoV Plpro/papain-like protease Protein (His)

SARS CoV ORF1ab polyprotein (PP1ab) is a 7073 residues long polyprotein which further produces 5 non-structural proteins (NSPs) after processing through virally encoded proteases (NSP12-NSP16). All these 5 proteins are mainly involved in viral replication, assembly, and immune response modulation. SARS-CoV Plpro/papain-like protease Protein (His) is the recombinant Virus-derived SARS-CoV Plpro/papain-like protease protein, expressed by E. coli , with C-His labeled tag. The total length of SARS-CoV Plpro/papain-like protease Protein (His) is 319 a.a., with molecular weight of ~36.91 kDa.

SARS CoV ORF1ab polyprotein (PP1ab) is a 7073 residues long polyprotein which further produces 5 non-structural proteins (NSPs) after processing through virally encoded proteases (NSP12-NSP16). All these 5 proteins are mainly involved in viral replication, assembly, and immune response modulation. Polyprotein degradation is mediated by main protease (Mpro) and papain-like protease (PLpro), PP1ab is thus processed into the individual NSPs that form the viral replication and transcription complex, The function of viral RNA transcription and replication is performed by a specific RNA dependent RNA polymerase (RdRp) region present in PP1ab ^[1][2].

Virus

E. coli

C-His

AAX16193 (E1541-Y1859)

/

Approximately 36.91 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as 0.22 μm filtered solution in 50 mM Tris, 0.1% Tween, 150 mM NaCl, pH 9.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.