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Semaphorin-3C/SEMA3C Protein, Human (HEK293, C-His)

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Based on 1 publication(s) in Google Scholar

Semaphorin-3C (SEMA3C) protein plays a crucial role in developmental processes by binding to plexin family members, particularly PLXND1, orchestrating intricate cellular signaling pathways. Its indispensable contribution to normal cardiovascular development during embryogenesis and its function as an attractant for growing axons underscore SEMA3C's significance in axon growth and guidance. Semaphorin-3C/SEMA3C Protein, Human (HEK293, C-His) is the recombinant human-derived Semaphorin-3C/SEMA3C, expressed by HEK293, with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Semaphorin-3C/SEMA3C Protein, Human (HEK293, C-His)
  • Semaphorin-3C/SEMA3C Protein, Human (HEK293, C-His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Semaphorin-3C (SEMA3C) protein plays a crucial role in developmental processes by binding to plexin family members, particularly PLXND1, orchestrating intricate cellular signaling pathways. Its indispensable contribution to normal cardiovascular development during embryogenesis and its function as an attractant for growing axons underscore SEMA3C's significance in axon growth and guidance. Semaphorin-3C/SEMA3C Protein, Human (HEK293, C-His) is the recombinant human-derived Semaphorin-3C/SEMA3C, expressed by HEK293, with C-6*His labeled tag.

Background

Semaphorin-3C (SEMA3C) protein serves as a pivotal regulator in developmental processes by binding to members of the plexin family. Crucially, SEMA3C is indispensable for normal cardiovascular development in embryogenesis. Its functionality extends to acting as an attractant for growing axons, thereby playing a significant role in axon growth and guidance. In molecular interactions, SEMA3C forms a specific association with PLXND1, highlighting its involvement in intricate cellular signaling pathways that contribute to the orchestration of developmental events.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells to Fibronectin. Recombinant Human Semaphorin 3C immobilized at 10 μg/mL, with 2 ng/mL rhFN-1, 100 μL/well, will induce approximately 40%-80% adhesion.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q99985-1 (G21-S738)

  • Gene ID
    10512
  • Protein Length

    Partial

  • Synonyms

    SEMA3C; Semaphorin-3C; Prev. SEMAE; Uncharacterized Protein SEMA3C; SemE; Semaphorin E; Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3C; Semaphorin-E; Semaphorin 3C; Sema E

  • AA Sequence

    GSSQPQARVYLTFDELRETKTSEYFSLSHHPLDYRILLMDEDQDRIYVGSKDHILSLNINNISQEALSVFWPASTIKVEECKMAGKDPTHGCGNFVRVIQTFNRTHLYVCGSGAFSPVCTYLNRGRRSEDQVFMIDSKCESGKGRCSFNPNVNTVSVMINEELFSGMYIDFMGTDAAIFRSLTKRNAVRTDQHNSKWLSEPMFVDAHVIPDGTDPNDAKVYFFFKEKLTDNNRSTKQIHSMIARICPNDTGGLRSLVNKWTTFLKARLVCSVTDEDGPETHFDELEDVFLLETDNPRTTLVYGIFTTSSSVFKGSAVCVYHLSDIQTVFNGPFAHKEGPNHQLISYQGRIPYPRPGTCPGGAFTPNMRTTKEFPDDVVTFIRNHPLMYNSIYPIHKRPLIVRIGTDYKYTKIAVDRVNAADGRYHVLFLGTDRGTVQKVVVLPTNNSVSGELILEELEVFKNHAPITTMKISSKKQQLYVSSNEGVSQVSLHRCHIYGTACADCCLARDPYCAWDGHSCSRFYPTGKRRSRRQDVRHGNPLTQCRGFNLKAYRNAAEIVQYGVKNNTTFLECAPKSPQASIKWLLQKDKDRRKEVKLNERIIATSQGLLIRSVQGSDQGLYHCIATENSFKQTIAKINFKVLDSEMVAVVTDKWSPWTWASSVRALPFHPKDIMGAFSHSEMQMINQYCKDTRQQHQQGDESQKMRGDYGKLKALINS

  • Predicted Molecular Mass

    81.4 kDa

  • Molecular Weight

    Approximately 65-72 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Semaphorin-3C/SEMA3C Protein, Human (HEK293, C-His) Product Image
    1/16
  • Experimental Validation Results for Semaphorin-3C/SEMA3C Protein, Human (HEK293, C-His)
  • Semaphorin-3C/SEMA3C Protein, Human (HEK293, C-His) Product Image