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Semaphorin-4D/SEMA4D Protein, Rat (HEK293, His)

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Based on 1 Customer Validation

Semaphorin-4D (SEMA4D) is predicted to participate in the same protein binding, signaling receptor binding, and transmembrane signaling receptor activities. Its multiple roles, including negative regulation of osteoblast differentiation and modulation of neuronal projection development, highlight its functional versatility. Semaphorin-4D/SEMA4D Protein, Rat (HEK293, His) is the recombinant rat-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Semaphorin-4D/SEMA4D Protein, Rat (HEK293, His)
  • Experimental Validation Results for Semaphorin-4D/SEMA4D Protein, Rat (HEK293, His)
  • Semaphorin-4D/SEMA4D Protein, Rat (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Semaphorin-4D (SEMA4D) is predicted to participate in the same protein binding, signaling receptor binding, and transmembrane signaling receptor activities. Its multiple roles, including negative regulation of osteoblast differentiation and modulation of neuronal projection development, highlight its functional versatility. Semaphorin-4D/SEMA4D Protein, Rat (HEK293, His) is the recombinant rat-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-His labeled tag.

Background

Semaphorin-4D (SEMA4D) is predicted to possess identical protein binding activity, signaling receptor binding activity, and transmembrane signaling receptor activity. Its involvement in various processes, including negative regulation of osteoblast differentiation, regulation of neuron projection development, and regulation of phosphate metabolic processes, underscores its diverse functions. SEMA4D is anticipated to act upstream of or within the positive regulation of axonogenesis, suggesting a role in neuronal development. The predicted activity in the extracellular space and its status as an integral component of the plasma membrane further emphasize its potential significance in intercellular communication. With biased expression observed in tissues such as the spleen, thymus, and nine other tissues, SEMA4D's intricate involvement in cellular processes and its potential impact on tissue-specific functions become areas of interest for further exploration.

Verified Bioactivity

Measured by its ability to inhibit survival of SK-OV-3 human ovarian carcinoma cells. The ED50 for this effect is 1.211 µg/mL, corresponding to a specific activity is 825.8 U/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit survival of SK-OV-3 human ovarian carcinoma cells. The ED50 for this effect is 1.211 µg/mL, corresponding to a specific activity is 825.8 U/mg.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-10*His
  • Accession

    A0A8I6G5Q1/NP_001164034 (F24-M712)

  • Gene ID
    306790  [NCBI]
  • Molecular Construction
    • N-term
    • SEMA4D (F24-M712)
      Accession # A0A8I6G5Q1/NP_001164034
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    SEMA4D; BB18; Prev. C9orf164; A8; Prev. SEMAJ; Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) And Short Cytoplasmic Domain, 4D; CD100; Chromosome 9 Open Reading Frame 164; Semaphorin-4D; CD100 Antigen; Coll-4; M-Sema G; Sema Domain, Im

  • AA Sequence

    FAPVPRITWEHGEVGLVKFHEPGIFNYSSLLMSEDKDTLYVGAREAVFAVNALDISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIHKSPDGTEAEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLSTVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFLSTDRGALHKAVILAKEVHVVEETQLFQDFEPVLSLLLSSKKGRKFVYAGSNSGVVQAPLAFCGKHSSCEDCVLARDPYCAWSPAIKACVTLHQAEGSSRGWIQDMSGDTSSCLDKSKESFHQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAVSPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHILEVKMVARIPPSPTSQTAQTEGSRITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSTGTSCEPKM

  • Molecular Weight

    Approximately 96 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit survival of SK-OV-3 human ovarian carcinoma cells. The ED50 for this effect is 1.211 µg/mL, corresponding to a specific activity is 825.8 U/mg.

  • Semaphorin-4D/SEMA4D Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Semaphorin-4D/SEMA4D Protein, Rat (HEK293, His)
  • Experimental Validation Results for Semaphorin-4D/SEMA4D Protein, Rat (HEK293, His)
  • Semaphorin-4D/SEMA4D Protein, Rat (HEK293, His) Product Image