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  3. Protease Inhibitors
  4. Serpin (Protease Inhibitor)
  5. Leukocyte Elastase Inhibitor
  6. Serpin B1 Protein, Human (HEK293, His)

Serpin B1 Protein, Human (HEK293, His)

Cat. No.: HY-P71303
COA Handling Instructions

Serpin B1 protein is an important neutrophil serine protease inhibitor that regulates innate immune responses by protecting cells from cytoplasmic proteases during stress. It inhibits various neutrophil proteases, including elastase and chymotrypsin, and blocks GZMH activity intracellularly. Serpin B1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin B1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Serpin B1 Protein, Human (HEK293, His) is 379 a.a., with molecular weight of ~44.0 kDa.

For research use only. We do not sell to patients.

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Description

Serpin B1 protein is an important neutrophil serine protease inhibitor that regulates innate immune responses by protecting cells from cytoplasmic proteases during stress. It inhibits various neutrophil proteases, including elastase and chymotrypsin, and blocks GZMH activity intracellularly. Serpin B1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin B1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Serpin B1 Protein, Human (HEK293, His) is 379 a.a., with molecular weight of ~44.0 kDa.

Background

The Serpin B1 protein, a neutrophil serine protease inhibitor, plays a crucial role in regulating the innate immune response, inflammation, and cellular homeostasis. Acting primarily to safeguard cells from proteases released in the cytoplasm during stress or infection, Serpin B1 protects against potential damage caused by the potent enzymes released from granules. It effectively regulates the activity of various neutrophil proteases, including elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Notably, Serpin B1 also serves as a potent intracellular inhibitor of GZMH, directly blocking its proteolytic activity. During inflammation, Serpin B1 limits the activity of inflammatory caspases (CASP1, CASP4, and CASP5) by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation. Moreover, when secreted, Serpin B1 promotes beta-cell proliferation through its protease inhibitory function. The protein functions as a monomer and interacts with CASP1, CASP4, CASP5, PRTN3, and GZMH, revealing its intricate role in modulating various cellular processes and interactions with key proteins in the immune and inflammatory pathways.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P30740 (M1-P379)

Gene ID
Molecular Construction
N-term
Serpin B1 (M1-P379)
Accession # P30740
6*His
C-term
Synonyms
Leukocyte elastase inhibitor; SERPINB1; Monocyte/neutrophil elastase inhibitor; M/NEI; Peptidase inhibitor 2; PI-2
AA Sequence

MEQLSSANTRFALDLFLALSENNPAGNIFISPFSISSAMAMVFLGTRGNTAAQLSKTFHFNTVEEVHSRFQSLNADINKRGASYILKLANRLYGEKTYNFLPEFLVSTQKTYGADLASVDFQHASEDARKTINQWVKGQTEGKIPELLASGMVDNMTKLVLVNAIYFKGNWKDKFMKEATTNAPFRLNKKDRKTVKMMYQKKKFAYGYIEDLKCRVLELPYQGEELSMVILLPDDIEDESTGLKKIEEQLTLEKLHEWTKPENLDFIEVNVSLPRFKLEESYTLNSDLARLGVQDLFNSSKADLSGMSGARDIFISKIVHKSFVEVNEEGTEAAAATAGIATFCMLMPEENFTADHPFLFFIRHNSSGSILFLGRFSSP

Molecular Weight

Approximately 44.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Serpin B1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Serpin B1 Protein, Human (HEK293, His)
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HY-P71303
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