Serpin B1 Protein, Human (HEK293, His)

Serpin B1 protein is an important neutrophil serine protease inhibitor that regulates innate immune responses by protecting cells from cytoplasmic proteases during stress. It inhibits various neutrophil proteases, including elastase and chymotrypsin, and blocks GZMH activity intracellularly. Serpin B1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin B1 protein, expressed by HEK293 , with C-6*His labeled tag.

The Serpin B1 protein, a neutrophil serine protease inhibitor, plays a crucial role in regulating the innate immune response, inflammation, and cellular homeostasis. Acting primarily to safeguard cells from proteases released in the cytoplasm during stress or infection, Serpin B1 protects against potential damage caused by the potent enzymes released from granules. It effectively regulates the activity of various neutrophil proteases, including elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Notably, Serpin B1 also serves as a potent intracellular inhibitor of GZMH, directly blocking its proteolytic activity. During inflammation, Serpin B1 limits the activity of inflammatory caspases (CASP1, CASP4, and CASP5) by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation. Moreover, when secreted, Serpin B1 promotes beta-cell proliferation through its protease inhibitory function. The protein functions as a monomer and interacts with CASP1, CASP4, CASP5, PRTN3, and GZMH, revealing its intricate role in modulating various cellular processes and interactions with key proteins in the immune and inflammatory pathways.

Human

HEK293

C-6*His

P30740-1 (M1-P379)

1992  [NCBI]

Full Length

MEQLSSANTRFALDLFLALSENNPAGNIFISPFSISSAMAMVFLGTRGNTAAQLSKTFHFNTVEEVHSRFQSLNADINKRGASYILKLANRLYGEKTYNFLPEFLVSTQKTYGADLASVDFQHASEDARKTINQWVKGQTEGKIPELLASGMVDNMTKLVLVNAIYFKGNWKDKFMKEATTNAPFRLNKKDRKTVKMMYQKKKFAYGYIEDLKCRVLELPYQGEELSMVILLPDDIEDESTGLKKIEEQLTLEKLHEWTKPENLDFIEVNVSLPRFKLEESYTLNSDLARLGVQDLFNSSKADLSGMSGARDIFISKIVHKSFVEVNEEGTEAAAATAGIATFCMLMPEENFTADHPFLFFIRHNSSGSILFLGRFSSP

Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.