SETD7 Protein, Human (sf9, GST)
The SETD7 protein is a histone methyltransferase that specifically monomethylates H3K4, a mark associated with transcriptional activation. Its role in gene activation extends to the collagenase and insulin genes, specifically at the insulin promoter when IPF1/PDX-1 is recruited. SETD7 Protein, Human (sf9, GST) is the recombinant human-derived SETD7 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The SETD7 protein is a histone methyltransferase that specifically monomethylates H3K4, a mark associated with transcriptional activation. Its role in gene activation extends to the collagenase and insulin genes, specifically at the insulin promoter when IPF1/PDX-1 is recruited. SETD7 Protein, Human (sf9, GST) is the recombinant human-derived SETD7 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
Background
SETD7 Protein, a histone methyltransferase, demonstrates specificity in monomethylating 'Lys-4' of histone H3. This methylation event serves as a distinctive mark associated with epigenetic transcriptional activation. SETD7 plays a pivotal role in the activation of transcription for genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, it leads to the activation of transcription. Beyond histones, SETD7 exhibits methyltransferase activity toward non-histone proteins, including CGAS, p53/TP53, TAF10, and possibly TAF7, recognizing and binding the [KR]-[STA]-K motif in substrate proteins. Monomethylation of 'Lys-189' of TAF10 enhances its affinity for RNA polymerase II. Moreover, SETD7 monomethylates 'Lys-372' of p53/TP53, stabilizing the protein and amplifying p53/TP53-mediated transcriptional activation. Additionally, it monomethylates 'Lys-491' of CGAS, facilitating interaction between SGF29 and CGAS (By similarity).
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
Q8WTS6/NP_085151.1 (M1-K366)
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Gene ID80854
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Molecular Construction
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N-term
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GST
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SETD7 (M1-K366)
Accession # Q8WTS6/NP_085151.1 -
C-term
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Protein Length
Full Length
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Synonyms
SETD7; KIAA1717; SET Domain Containing 7, Histone Lysine Methyltransferase; Set9; SET7/9; SET Domain Containing Lysine Methyltransferase 7; SET7; Lysine N-Methyltransferase 7; KMT7; H3-K4-HMTase SETD7; Histone-Lysine N-Methyltransferase SETD7; SET Domain
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AA Sequence
MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK
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Molecular Weight
Approximately 75 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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