SLC11A1 Protein, Human (HEK293, His-MBP, FLAG)
The SLC11A1 protein is a macrophage-specific antiporter that transports metal ions reversibly through proton gradients. It localizes to late endosomal lysosomal membranes and transports divalent cations into acidic compartments, affecting antibacterial activity. SLC11A1 Protein, Human (HEK293, His-MBP, FLAG) is the recombinant human-derived SLC11A1 protein, expressed by sHEK293 , with N-8*His-MBP, C-Flag labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The SLC11A1 protein is a macrophage-specific antiporter that transports metal ions reversibly through proton gradients. It localizes to late endosomal lysosomal membranes and transports divalent cations into acidic compartments, affecting antibacterial activity. SLC11A1 Protein, Human (HEK293, His-MBP, FLAG) is the recombinant human-derived SLC11A1 protein, expressed by sHEK293 , with N-8*His-MBP, C-Flag labeled tag.
Background
SLC11A1 Protein, a macrophage-specific antiporter, facilitates bidirectional metal ion transport against a proton gradient. Positioned on late endosomal lysosomal membranes, it transfers bivalent cations from the cytosol into these acidic compartments, impacting antimicrobial activity directly. Notably involved in iron metabolism, it plays a crucial role in the host's innate resistance to intracellular parasites. This resistance mechanism involves sequestering Fe(2+) and Mn(2+), essential cofactors for both prokaryotic and eukaryotic catalases and superoxide dismutases. The sequestration serves a dual purpose, protecting macrophages from reactive oxygen species and denying pathogens the essential cations for synthesizing their protective enzymes (Probable).
Technical Parameters
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Species Human
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Source HEK293
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Tag N-8*His;C-Flag;N-MBP
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Accession
P49279 (T2-G550)
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Gene ID6556
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Molecular Construction
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N-term
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8*His-MBP
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SLC11A1 (T2-G550)
Accession # P49279 -
Flag
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C-term
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Protein Length
Full Length
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Synonyms
SLC11A1; Solute Carrier Family 11 (Proton-Coupled Divalent Metal Ion Transporter), Member 1; Prev. NRAMP1; NRAMP 1; Prev. NRAMP; Solute Carrier Family 11 (Proton-Coupled Divalent Metal Ion Transporters), Member 1; Prev. LSH; Solute Carrier Family 11 (Sodi
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AA Sequence
TGDKGPQRLSGSSYGSISSPTSPTSPGPQQAPPRETYLSEKIPIPDTKPGTFSLRKLWAFTGPGFLMSIAFLDPGNIESDLQAGAVAGFKLLWVLLWATVLGLLCQRLAARLGVVTGKDLGEVCHLYYPKVPRTVLWLTIELAIVGSDMQEVIGTAIAFNLLSAGRIPLWGGVLITIVDTFFFLFLDNYGLRKLEAFFGLLITIMALTFGYEYVVARPEQGALLRGLFLPSCPGCGHPELLQAVGIVGAIIMPHNIYLHSALVKSREIDRARRADIREANMYFLIEATIALSVSFIINLFVMAVFGQAFYQKTNQAAFNICANSSLHDYAKIFPMNNATVAVDIYQGGVILGCLFGPAALYIWAIGLLAAGQSSTMTGTYAGQFVMEGFLRLRWSRFARVLLTRSCAILPTVLVAVFRDLRDLSGLNDLLNVLQSLLLPFAVLPILTFTSMPTLMQEFANGLLNKVVTSSIMVLVCAINLYFVVSYLPSLPHPAYFGLAALLAAAYLGLSTYLVWTCCLAHGATFLAHSSHHHFLYGLLEEDQKGETSG
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Predicted Molecular Mass
103.6 kDa
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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