SLC11A2 Protein, Human (HEK293, His, MBP, FLAG)
The SLC11A2 protein is a proton-coupled metal ion symporter that selectively transports divalent metal cations, including Cd(2+), Fe(2+), Co(2+), Mn(2+), and less Significant Zn(2+), Ni(2+), VO(2+), the stoichiometric ratio of protons and metal ions is 1:1. It is critical for iron homeostasis, regulating intestinal Fe(2+) absorption, transporting TF-associated endosomal Fe(2+), and promoting Fe(2+) and Mn(2+) entry into mitochondria. SLC11A2 Protein, Human (HEK293, His, MBP, FLAG) is the recombinant human-derived SLC11A2 protein, expressed by HEK293 , with N-MBP, C-Flag, N-8*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The SLC11A2 protein is a proton-coupled metal ion symporter that selectively transports divalent metal cations, including Cd(2+), Fe(2+), Co(2+), Mn(2+), and less Significant Zn(2+), Ni(2+), VO(2+), the stoichiometric ratio of protons and metal ions is 1:1. It is critical for iron homeostasis, regulating intestinal Fe(2+) absorption, transporting TF-associated endosomal Fe(2+), and promoting Fe(2+) and Mn(2+) entry into mitochondria. SLC11A2 Protein, Human (HEK293, His, MBP, FLAG) is the recombinant human-derived SLC11A2 protein, expressed by HEK293 , with N-MBP, C-Flag, N-8*His labeled tag.
Background
The SLC11A2 protein, a proton-coupled metal ion symporter, operates with a proton to metal ion stoichiometry of 1:1, selectively transporting various divalent metal cations with varying affinities, including Cd(2+), Fe(2+), Co(2+), Mn(2+), and less significantly Zn(2+), Ni(2+), VO(2+). It plays a crucial role in maintaining iron homeostasis by modulating the intestinal absorption of dietary Fe(2+) and facilitating the transport of TF-associated endosomal Fe(2+) in erythroid precursors and other cells. Additionally, SLC11A2 facilitates the entry of Fe(2+) and Mn(2+) ions into mitochondria, suggesting its involvement in promoting mitochondrial heme synthesis, iron-sulfur cluster biogenesis, and antioxidant defense. Notably, the protein exhibits the ability to mediate uncoupled fluxes of either protons or metal ions, showcasing its versatile transport capabilities.
Technical Parameters
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Species Human
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Source HEK293
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Tag N-MBP;C-Flag;N-8*His
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Accession
P49281 (V2-R568)
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Gene ID4891
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Molecular Construction
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N-term
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8*His-MBP
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SLC11A2 (V2-R568)
Accession # P49281 -
Flag
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C-term
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Protein Length
Full Length
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Synonyms
SLC11A2; Divalent Metal Transporter 1; Prev. NRAMP2; NRAMP 2; Natural Resistance-Associated Macrophage Protein 2; CDNA FLJ46799 Fis, Clone TRACH3031678, Highly Similar To Natural Resistance-Associated Macrophage Protein 2; DMT-1; Solute Carrier Family 11
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AA Sequence
VLGPEQKMSDDSVSGDHGESASLGNINPAYSNPSLSQSPGDSEEYFATYFNEKISIPEEEYSCFSFRKLWAFTGPGFLMSIAYLDPGNIESDLQSGAVAGFKLLWILLLATLVGLLLQRLAARLGVVTGLHLAEVCHRQYPKVPRVILWLMVELAIIGSDMQEVIGSAIAINLLSVGRIPLWGGVLITIADTFVFLFLDKYGLRKLEAFFGFLITIMALTFGYEYVTVKPSQSQVLKGMFVPSCSGCRTPQIEQAVGIVGAVIMPHNMYLHSALVKSRQVNRNNKQEVREANKYFFIESCIALFVSFIINVFVVSVFAEAFFGKTNEQVVEVCTNTSSPHAGLFPKDNSTLAVDIYKGGVVLGCYFGPAALYIWAVGILAAGQSSTMTGTYSGQFVMEGFLNLKWSRFARVVLTRSIAIIPTLLVAVFQDVEHLTGMNDFLNVLQSLQLPFALIPILTFTSLRPVMSDFANGLGWRIAGGILVLIICSINMYFVVVYVRDLGHVALYVVAAVVSVAYLGFVFYLGWQCLIALGMSFLDCGHTCHLGLTAQPELYLLNTMDADSLVSR
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Predicted Molecular Mass
106 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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