SLC15A4 Protein, Human (HEK293, His)

SLC15A4 Protein, Human (HEK293, His) is the recombinant human-derived SLC15A4, expressed by HEK293 , with His labeled tag.

SLC15A4 is a proton-coupled amino acid transporter that facilitates the transmembrane transport of L-histidine and select di- and tripeptides from lysosomes to the cytosol, playing a pivotal role in innate immunity. It transports diverse peptides, including carnosine and certain peptidoglycans, with pH-dependent activity maximized in acidic lysosomal environments (By similarity). The protein contributes to microbial pathogen detection via Toll-like receptors (TLRs) and NOD-like receptors (NLRs), likely by shuttling bacterial peptidoglycans (e.g., NOD2 ligand muramyl dipeptide [MDP] and NOD1 ligand tri-DAP) across endolysosomal membranes. In plasmacytoid dendritic cells (pDCs), it is essential for TLR7/8/9-mediated type I interferon (IFN-I) production. Independently of transport function, SLC15A4 recruits the innate immune adapter TASL to endolysosomes downstream of TLR7/8/9, enabling IRF5 activation. It also supports TLR9-stimulated class switch recombination to IgG2c (By similarity) and regulates mast cell secretory-granule homeostasis by restraining inflammatory responses (By similarity).

Human

HEK293

C-6*His

Q8N697-1 (M1-A577)

121260

Full Length of Isoform-1

MEGSGGGAGERAPLLGARRAAAAAAAAGAFAGRRAACGAVLLTELLERAAFYGITSNLVLFLNGAPFCWEGAQASEALLLFMGLTYLGSPFGGWLADARLGRARAILLSLALYLLGMLAFPLLAAPATRAALCGSARLLNCTAPGPDAAARCCSPATFAGLVLVGLGVATVKANITPFGADQVKDRGPEATRRFFNWFYWSINLGAILSLGGIAYIQQNVSFVTGYAIPTVCVGLAFVVFLCGQSVFITKPPDGSAFTDMFKILTYSCCSQKRSGERQSNGEGIGVFQQSSKQSLFDSCKMSHGGPFTEEKVEDVKALVKIVPVFLALIPYWTVYFQMQTTYVLQSLHLRIPEISNITTTPHTLPAAWLTMFDAVLILLLIPLKDKLVDPILRRHGLLPSSLKRIAVGMFFVMCSAFAAGILESKRLNLVKEKTINQTIGNVVYHAADLSLWWQVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFFFSGVGSFVGSGLLALVSIKAIGWMSSHTDFGNINGCYLNYYFFLLAAIQGATLLLFLIISVKYDHHRDHQRSRANGVPTSRRA

Approximately 50 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 25 mM HEPES, pH 7.5, 300 mM NaCl, 2 mM DTT, 0.01% (w/v) LMNG, 0.001% (w/v) CHS.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.