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SLC44A1 Protein, Human (sf9, His, MBP, FLAG)

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The SLC44A1 protein acts as a choline/H+ and high-affinity ethanolamine/H+ antiporter and is critical for regulating cellular choline and ethanolamine transport. Its dual function actively redistributes intracellular ethanolamine and balances the CDP-Cho and CDP-Etn arms of the Kennedy pathway. SLC44A1 Protein, Human (sf9, His, MBP, FLAG) is the recombinant human-derived SLC44A1 protein, expressed by sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • SLC44A1 Protein, Human (sf9, His, MBP, FLAG) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The SLC44A1 protein acts as a choline/H+ and high-affinity ethanolamine/H+ antiporter and is critical for regulating cellular choline and ethanolamine transport. Its dual function actively redistributes intracellular ethanolamine and balances the CDP-Cho and CDP-Etn arms of the Kennedy pathway. SLC44A1 Protein, Human (sf9, His, MBP, FLAG) is the recombinant human-derived SLC44A1 protein, expressed by sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag.

Background

SLC44A1 protein functions as a choline/H+ antiporter, playing a pivotal role in the regulation of cellular choline transport as documented in various studies. Additionally, it acts as a high-affinity ethanolamine/H+ antiporter, thereby controlling the supply of extracellular ethanolamine for the CDP-Etn pathway. This dual functionality allows SLC44A1 to actively redistribute intracellular ethanolamine, maintaining a balance between the CDP-Cho and CDP-Etn arms of the Kennedy pathway. The involvement of SLC44A1 in these processes is crucial for membrane synthesis and myelin production, highlighting its significance in fundamental cellular functions and suggesting potential implications for membrane homeostasis and neural development.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-MBP;C-Flag;N-8*His
  • Accession

    Q8WWI5 (G2-A657)

  • Gene ID
    23446
  • Molecular Construction
    • N-term
    • 8*His-MBP
    • SLC44A1 (G2-A657)
      Accession # Q8WWI5
    • Flag
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    SLC44A1; Solute Carrier Family 44 (Choline Transporter), Member 1; Prev. CDW92; Choline Transporter-Like Protein; CTL1; CD92 Antigen; CD92; CONATOC; Choline Transporter-Like Protein 1; CDw92; CHTL1; Solute Carrier Family 44 Member 1; CDW92 Antigen

  • AA Sequence

    GCCSSASSAAQSSKREWKPLEDRSCTDIPWLLLFILFCIGMGFICGFSIATGAAARLVSGYDSYGNICGQKNTKLEAIPNSGMDHTQRKYVFFLDPCNLDLINRKIKSVALCVAACPRQELKTLSDVQKFAEINGSALCSYNLKPSEYTTSPKSSVLCPKLPVPASAPIPFFHRCAPVNISCYAKFAEALITFVSDNSVLHRLISGVMTSKEIILGLCLLSLVLSMILMVIIRYISRVLVWILTILVILGSLGGTGVLWWLYAKQRRSPKETVTPEQLQIAEDNLRALLIYAISATVFTVILFLIMLVMRKRVALTIALFHVAGKVFIHLPLLVFQPFWTFFALVLFWVYWIMTLLFLGTTGSPVQNEQGFVEFKISGPLQYMWWYHVVGLIWISEFILACQQMTVAGAVVTYYFTRDKRNLPFTPILASVNRLIRYHLGTVAKGSFIITLVKIPRMILMYIHSQLKGKENACARCVLKSCICCLWCLEKCLNYLNQNAYTATAINSTNFCTSAKDAFVILVENALRVATINTVGDFMLFLGKVLIVCSTGLAGIMLLNYQQDYTVWVLPLIIVCLFAFLVAHCFLSIYEMVVDVLFLCFAIDTKYNDGSPGREFYMDKVLMEFVENSRKAMKEAGKGGVADSRELKPMASGASSA

  • Predicted Molecular Mass

    117.0 kDa

  • Purity

    ≥ 80%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 150 mM NaCl, 0.03% (w/v) DDM, 0.003% (w/v) CHS.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • SLC44A1 Protein, Human (sf9, His, MBP, FLAG) Product Image