SOCS3 Protein, Human (His-Trx)

SOCS3 is a member of the SOCS family that operates in a negative feedback loop to regulate cytokine signaling, particularly in the JAK/STAT pathway. It inhibits cytokine signaling by binding to receptors such as IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF, and leptin receptors. SOCS3 Protein, Human (His-Trx) is the recombinant human-derived SOCS3 protein, expressed by E. coli , with N-Trx, N-His labeled tag. The total length of SOCS3 Protein, Human (His-Trx) is 225 a.a., with molecular weight of ~46 kDa.

SOCS3, a member of the SOCS family, operates within a classical negative feedback system that intricately regulates cytokine signal transduction. Particularly, SOCS3 exerts its influence on the JAK/STAT pathway, engaging in the negative regulation of cytokines. By binding to tyrosine kinase receptors such as IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF, and leptin receptors, SOCS3 effectively inhibits cytokine signal transduction. Notably, its interaction with JAK2 hampers the kinase activity of the latter, thereby modulating IL6 signaling. Beyond its role in signal transduction, SOCS3 plays a critical part in suppressing fetal liver erythropoiesis and regulating the initiation and sustenance of allergic responses mediated by T-helper type 2 cells. Moreover, it serves as a probable substrate recognition component within a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex, facilitating the ubiquitination and subsequent proteasomal degradation of target proteins—an aspect integral to protein modification and ubiquitination processes.

Human

E. coli

N-Trx;N-His

O14543 (M1-L225)

9021  [NCBI]

Approximately 46 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.