SOD3 Protein, Human (His)
Based on 1 Customer Validation
SOD3 Protein, Human (His) is the recombinant human-derived SOD3 protein, expressed by E. coli, with N-6*His tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
SOD3 Protein, Human (His) is the recombinant human-derived SOD3 protein, expressed by E. coli, with N-6*His tag.
Background
SOD3 is an enzyme that protects the extracellular space from the toxic effects of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P08294 (W19-A240)
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Gene ID6649
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Molecular Construction
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N-term
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6*His
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SOD3 (W19-A240)
Accession # P08294 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
SOD3; Superoxide Dismutase 3, Extracellular; Superoxide Dismutase 3; Testicular Tissue Protein Li 175; EC-SOD; Superoxide Dismutase [Cu-Zn]; Extracellular Superoxide Dismutase [Cu-Zn]
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AA Sequence
WTGEDSAEPNSDSAEWIRDMYAKVTEIWQEVMQRRDDDGALHAACQVQPSATLDAAQPRVTGVVLFRQLAPRAKLDAFFALEGFPTEPNSSSRAIHVHQFGDLSQGCESTGPHYNPLAVPHPQHPGDFGNFAVRDGSLWRYRAGLAASLAGPHSIVGRAVVVHAGEDDLGRGGNQASVENGNAGRRLACCVVGVCGPGLWERQAREHSERKKRRRESECKAA
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Predicted Molecular Mass
28.1 kDa
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Molecular Weight
Approximately 30 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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