SP-17 Protein, Mouse (His)
Based on 1 Customer Validation
SP-17 protein acts as a sperm surface zona pellucida binding protein, facilitating high-affinity spermatozoa binding to the zona pellucida.It potentially engages in binding interactions with zona pellucida and carbohydrates.Existing as a homodimer, SP-17 may interact with ROPN1, suggesting a cooperative role in sperm-egg interactions and fertilization processes.SP-17 Protein, Mouse (His) is the recombinant mouse-derived SP-17 protein, expressed by E.coli , with N-His labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
SP-17 protein acts as a sperm surface zona pellucida binding protein, facilitating high-affinity spermatozoa binding to the zona pellucida.It potentially engages in binding interactions with zona pellucida and carbohydrates.Existing as a homodimer, SP-17 may interact with ROPN1, suggesting a cooperative role in sperm-egg interactions and fertilization processes.SP-17 Protein, Mouse (His) is the recombinant mouse-derived SP-17 protein, expressed by E.coli , with N-His labeled tag.
Background
The SP-17 protein serves as a sperm surface zona pellucida binding protein, facilitating the high-affinity binding of spermatozoa to the zona pellucida. Its potential role includes participation in the binding interactions with zona pellucida and carbohydrates. Existing as a homodimer, SP-17 may also engage in interactions with ROPN1, indicating a possible cooperative role in the processes related to sperm-egg interactions and fertilization.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-8*His
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Accession
Q62252 (M1-N149)
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Molecular Construction
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N-term
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8*His
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SP-17 (M1-N149)
Accession # Q62252 -
C-term
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Protein Length
Full Length
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Synonyms
SPA17; Cancer/Testis Antigen 22; Sperm Autoantigenic Protein 17; Sperm Protein 17; SP17; SP17-1; CT22; Sp17-1; Sperm Surface Protein Sp17
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AA Sequence
MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFENLLEKREKTSFDPAEWGAKVEDRFYNNHAFKEQEQVEKCEQELAKSSGREETPVTPFEESTEEEREQEEAAALKIQSLFRGHVAREEVKKMKSDKNENLKEEADN
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Predicted Molecular Mass
18.4 kDa
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Molecular Weight
Approximately 20-25 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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