SULT2B1 Protein, Human (311a.a, C-His)

The SULT2B1 protein uses PAPS to catalyze sulfate conjugation, mainly sulfating cholesterol, and shows significant activity on steroids such as pregnenolone and DHEA.It plays a crucial role in epidermal cholesterol metabolism, regulating epidermal proliferation and differentiation.SULT2B1 Protein, Human (C-His) is the recombinant human-derived SULT2B1 protein, expressed by E.coli , with C-6*His labeled tag.

SULT2B1 protein, a sulfotransferase utilizing 3'-phospho-5'-adenylyl sulfate (PAPS) as its sulfonate donor, holds a pivotal role in catalyzing sulfate conjugation, with a primary responsibility for sulfating cholesterol. Additionally, SULT2B1 demonstrates significant activity in sulfating steroids, particularly the 3beta-hydroxyl groups of molecules such as pregnenolone and dehydroepiandrosterone (DHEA). While it exhibits a preferential sulfonation of cholesterol, it also displays substantial activity with pregnenolone and DHEA. Beyond its enzymatic functions, SULT2B1 plays a crucial role in epidermal cholesterol metabolism, contributing to the regulation of epidermal proliferation and differentiation. Notably, it sulfonates pregnenolone but not cholesterol, underscoring its substrate selectivity and emphasizing its multifaceted involvement in the intricate processes governing cholesterol homeostasis and steroid metabolism.

Measured by its ability to transfer sulfate from PAPS to pregnenolone. The specific activity is 17.167 pmol/min/μg that incubate at 37 °C for 30 minutes.

Human

E. coli

C-6*His

O00204-1 (M1-E311)

6820

Partial

MDGPAEPQIPGLWDTYEDDISEISQKLPGEYFRYKGVPFPVGLYSLESISLAENTQDVRDDDIFIITYPKSGTTWMIEIICLILKEGDPSWIRSVPIWERAPWCETIVGAFSLPDQYSPRLMSSHLPIQIFTKAFFSSKAKVIYMGRNPRDVVVSLYHYSKIAGQLKDPGTPDQFLRDFLKGEVQFGSWFDHIKGWLRMKGKDNFLFITYEELQQDLQGSVERICGFLGRPLGKEALGSVVAHSTFSAMKANTMSNYTLLPPSLLDHRRGAFLRKGVCGDWKNHFTVAQSEAFDRAYRKQMRGMPTFPWDE

Approximately 37 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.0, 8% trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.