T-PA Protein, Human (HEK293)

The T-PA protein encoded by this gene is a secreted serine protease that converts plasminogen into plasmin, a key enzyme involved in tissue remodeling and cell migration. The proteolytic process produces heavy and light chains, forming heterodimeric enzymes. T-PA Protein, Human (HEK293) is the recombinant human-derived T-PA protein, expressed by HEK293 , with tag free.

T-PA Protein, encoded by this gene, serves as a secreted serine protease crucial for the conversion of the proenzyme plasminogen to plasmin, a key fibrinolytic enzyme involved in tissue remodeling and cell migration. The preproprotein undergoes proteolytic processing by plasmin or trypsin, generating heavy and light chains that associate via disulfide linkages to form the heterodimeric enzyme. T-PA's enzymatic activity plays a pivotal role in maintaining the delicate balance between fibrinolysis and coagulation. Increased activity leads to hyperfibrinolysis, resulting in excessive bleeding, while decreased activity can lead to hypofibrinolysis, potentially causing thrombosis or embolism. Alternative splicing produces multiple transcript variants, including at least one isoform that undergoes proteolytic processing. The gene exhibits broad expression, with notable levels observed in the urinary bladder (RPKM 61.1), placenta (RPKM 41.3), and 18 other tissues, reflecting its diverse roles in various physiological contexts.

Measured by its ability to cleave a peptide substrate, Z-Gly-Gly-Arg-AMC. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read). The specific activity is 3403.37 pmol/min/µg, as measured under the described conditions.

Human

HEK293

Tag Free

NP_000921.1 (I311-P562)

5327  [NCBI]

Partial

IKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP

Approximately 35 kDa

• 
  ≥ 80%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 10 mM NaCl, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.