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T4 DNA Polymerase Protein, T4 phage (His)

T4 DNA Polymerase, a pivotal enzyme in viral DNA replication, performs dual functions crucial for genome synthesis. As a polymerase, it synthesizes DNA, and with its exonucleolytic activity, it proofreads by degrading single-stranded DNA in the 3'- to 5'-direction, ensuring the accuracy and fidelity of replicated viral genomic DNA. T4 DNA Polymerase Protein, T4 phage (His) is the recombinant T4 DNA Polymerase protein, expressed by E. coli , with N-6*His labeled tag. T4 DNA Polymerase Protein, T4 phage (His), has molecular weight of ~103.6 kDa.

For research use only. We do not sell to patients.
  • Species: Others
  • Source: E. coli
  • Storage:
    Stored at -20°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -20°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

T4 DNA Polymerase, a pivotal enzyme in viral DNA replication, performs dual functions crucial for genome synthesis. As a polymerase, it synthesizes DNA, and with its exonucleolytic activity, it proofreads by degrading single-stranded DNA in the 3'- to 5'-direction, ensuring the accuracy and fidelity of replicated viral genomic DNA. T4 DNA Polymerase Protein, T4 phage (His) is the recombinant T4 DNA Polymerase protein, expressed by E. coli , with N-6*His labeled tag. T4 DNA Polymerase Protein, T4 phage (His), has molecular weight of ~103.6 kDa.

Background

T4 DNA Polymerase, a key enzyme in viral DNA replication, exhibits dual enzymatic functions critical for its role in genome synthesis. This polymerase showcases both DNA synthesis, where it acts as a polymerase, and exonucleolytic activity, facilitating the degradation of single-stranded DNA in the 3'- to 5'-direction. The latter function serves a proofreading purpose, ensuring the accuracy and fidelity of the replicated viral genomic DNA.

Technical Parameters

  • Species Others
  • Source E. coli
  • Tag N-6*His
  • Accession

    P04415 Sequence information is not available or disclosed for this product.

  • Gene ID
    1258685  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • T4 DNA Polymerase
      Accession # P04415
    • C-term
  • Synonyms

    DNA-directed DNA polymerase; EC:2.7.7.7; EC:3.1.11.-; Gene product 43 (Gp43); 43

  • AA Sequence

    MKEFYISIETVGNNIVERYIDENGKERTREVEYLPTMFRHCKEESKYKDIYGKNCAPQKFPSMKDARDWMKRMEDIGLEALGMNDFKLAYISDTYGSEIVYDRKFVRVANCDIEVTGDKFPDPMKAEYEIDAITHYDSIDDRFYVFDLLNSMYGSVSKWDAKLAAKLDCEGGDEVPQEILDRVIYMPFDNERDMLMEYINLWEQKRPAIFTGWNIEGFDVPYIMNRVKMILGERSMKRFSPIGRVKSKLIQNMYGSKEIYSIDGVSILDYLDLYKKFAFTNLPSFSLESVAQHETKKGKLPYDGPINKLRETNHQRYISYNIIDVESVQAIDKIRGFIDLVLSMSYYAKMPFSGVMSPIKTWDAIIFNSLKGEHKVIPQQGSHVKQSFPGAFVFEPKPIARRYIMSFDLTSLYPSIIRQVNISPETIRGQFKVHPIHEYIAGTAPKPSDEYSCSPNGWMYDKHQEGIIPKEIAKVFFQRKDWKKKMFAEEMNAEAIKKIIMKGAGSCSTKPEVERYVKFSDDFLNELSNYTESVLNSLIEECEKAATLANTNQLNRKILINSLYGALGNIHFRYYDLRNATAITIFGQVGIQWIARKINEYLNKVCGTNDEDFIAAGDTDSVYVCVDKVIEKVGLDRFKEQNDLVEFMNQFGKKKMEPMIDVAYRELCDYMNNREHLMHMDREAISCPPLGSKGVGGFWKAKKRYALNVYDMEDKRFAEPHLKIMGMETQQSSTPKAVQEALEESIRRILQEGEESVQEYYKNFEKEYRQLDYKVIAEVKTANDIAKYDDKGWPGFKCPFHIRGVLTYRRAVSGLGVAPILDGNKVMVLPLREGNPFGDKCIAWPSGTELPKEIRSDVLSWIDHSTLFQKSFVKPLAGMCESAGMDYEEKASLDFLFG

  • Molecular Weight

    Approximately 104 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Storage & Stability

Stored at -20°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -20°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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