TFRC Protein, Cynomolgus (Biotinylated, HEK293, His)
TFRC proteins mediate iron uptake by triggering receptor-mediated endocytosis of ligand-occupied transferrin receptors, followed by acidification and iron release. It is essential for red blood cell and nervous system development, and for maintaining iron balance. TFRC Protein, Cynomolgus (Biotinylated, HEK293, His) is the recombinant cynomolgus-derived TFRC protein, expressed by HEK293 , with N-His labeled tag.
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
TFRC proteins mediate iron uptake by triggering receptor-mediated endocytosis of ligand-occupied transferrin receptors, followed by acidification and iron release. It is essential for red blood cell and nervous system development, and for maintaining iron balance. TFRC Protein, Cynomolgus (Biotinylated, HEK293, His) is the recombinant cynomolgus-derived TFRC protein, expressed by HEK293 , with N-His labeled tag.
Background
TFRC protein orchestrates the cellular uptake of iron through receptor-mediated endocytosis of ligand-occupied transferrin receptors into specialized endosomes. This process involves endosomal acidification, leading to the release of iron, followed by the recycling of the apotransferrin-receptor complex to the cell surface with a return to neutral pH, concomitant with the loss of apotransferrin affinity for its receptor. Essential for the development of erythrocytes and the nervous system, TFRC serves a critical role in iron homeostasis. Beyond its iron-related functions, TFRC acts as a lipid sensor, regulating mitochondrial fusion by modulating the activation of the JNK pathway. Furthermore, TFRC forms homodimers through disulfide linkages, adding another layer to its multifaceted involvement in cellular processes.
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag N-His
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Accession
G8F602 (C89-F760)
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Gene ID/
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Molecular Construction
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N-term
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His
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TFRC (C89-F760)
Accession # G8F602 -
C-term
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Protein Length
Extracellular Domain
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Conjugation
Biotin
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Conjugate Method
The biotin is covalently conjugated to the primary amine groups of the protein using chemical labeling methods.
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Synonyms
TFRC; Transferrin Receptor (P90, CD71); Transferrin Receptor; CD71 Antigen; P90; IMD46; Transferrin Receptor Protein 1; TRFR; CD71; Trfr; TFR1; TfR1; Transferrin Receptor 1; TFR; T9; TfR; TR
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AA Sequence
CKGVEPKTECERLAGTESPAREEPEEDFPAAPRLYWDDLKRKLSEKLDTTDFTSTIKLLNENLYVPREAGSQKDENLALYIENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGGLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLDSPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVKADLSFFGHAHLGTGDPYTPGFPSFNHTQFPPSQSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCKMVTSENKSVKLTVSNVLKETKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSSVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQDVKHPVTGRSLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELVERIPELNKVARAAAEVAGQFVIKLTHDTELNLDYERYNSQLLLFLRDLNQYRADVKEMGLSLQWLYSARGDFFRATSRLTTDFRNAEKRDKFVMKKLNDRVMRVEYYFLSPYVSPKESPFRHVFWGSGSHTLSALLESLKLRRQNNSAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF
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Predicted Molecular Mass
77.1 kDa
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Molecular Weight
Approximately 70-90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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