TFRC Protein, Cynomolgus (HEK293, His)

TFRC is a key protein in cellular iron uptake, coordinating receptor-mediated endocytosis of the ligand transferrin into specialized endosomes, where endosomal acidification triggers iron release. TFRC actively regulates T and B cell proliferation through iron uptake. TFRC Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived TFRC protein, expressed by HEK293 , with N-His labeled tag. The total length of TFRC Protein, Cynomolgus (HEK293, His) is 672 a.a., with molecular weight of 78-80 kDa.

TFRC, a pivotal protein in cellular iron uptake, orchestrates receptor-mediated endocytosis of ligand-occupied transferrin into specialized endosomes, where endosomal acidification triggers iron release. The apotransferrin-receptor complex undergoes recycling to the cell surface while a return to neutral pH, leading to the loss of apotransferrin affinity for its receptor. Essential for erythrocyte and nervous system development, TFRC positively regulates T and B cell proliferation through iron uptake. Acting as a lipid sensor, TFRC modulates mitochondrial fusion by regulating the JNK pathway. Low dietary stearate levels induce JNK pathway activation, resulting in HUWE1-mediated ubiquitination and degradation of mitofusin MFN2, inhibiting mitochondrial fusion. Conversely, high stearate levels stearoylate TFRC, suppressing JNK pathway activation and preventing MFN2 degradation. TFRC also can function as a homodimer with disulfide linkage, binding one transferrin molecule per subunit, and interacts with SH3BP4 and STEAP3, the latter facilitating TFRC endocytosis in erythroid precursor cells.

Cynomolgus

HEK293

N-His

XP_005545315 (C89-F760)

102136007  [NCBI]

78-80 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.