TIM-3/HAVCR2 Protein, Human (HEK293, Fc)
Based on 1 Customer Validation
TIM3 Protein, Human (HEK293, Fc) is a type I transmembrane glycoprotein belonging to T Cell Immunoglobulin Mucin (TIM) family, and is involved in regulating innate and adaptive immunity.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
TIM3 Protein, Human (HEK293, Fc) is a type I transmembrane glycoprotein belonging to T Cell Immunoglobulin Mucin (TIM) family, and is involved in regulating innate and adaptive immunity.
Background
T Cell Immunoglobulin Mucin 3 (TIM-3), also known as Hepatitis A virus cellular receptor 2 (HAVCR2), is an immunomodulatory molecule and upregulated in T cells by several cytokines. TIM-3 also influences mast cell function but its transcriptional regulation in mast cells has not been clarified. Human TIM-3 consists of a membrane distal variable immunoglobulin (IgV)-like domain involved in ligand interactions, a membrane proximal mucin domain and a transmembrane domain that is connected to an intracellular cytoplasmic tail involved in phosphotyrosine-dependent signaling[1][2][3].
Verified Bioactivity
1.Immobilized TIM-3, hFc, Human at 0.5 μg/mL, the concentration of Anti-TIM3 mouse antibody (Genscript) that produces 50% optimal binding response is found to be approximately 5.0 ng/mL.
2.Immobilized Galectin-9, His, Human at 0.5 μg/mL (100 µL/well) can bind TIM-3, hFc, Human with a linear range of 0.78-6.25 μg/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-hFc
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Accession
Q8TDQ0-1 (S22-R200)
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Molecular Construction
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N-term
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TIM-3 (S22-R200)
Accession # Q8TDQ0-1 -
hFc
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C-term
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Protein Length
Extracellular Domain
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Synonyms
HAVCR2; FLJ14428; Hepatitis A Virus Cellular Receptor 2; HAVcr-2; TIMD3; TIMD-3; TIM3; T-Cell Immunoglobulin And Mucin Domain 3; Tim-3; T Cell Immunoglobulin Mucin 3; CD366; Kidney Injury Molecule-3; T-Cell Immunoglobulin And Mucin Domain-Containing Prote
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AA Sequence
SEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIR
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Predicted Molecular Mass
46.5 kDa
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Molecular Weight
Approximately 60-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS.
<0.2 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Homayouni V, et al. Stimulation of Camel Polyclonal Antibody against Human T cell Immunoglobulin and Mucin 3. Iran J Biotechnol. 2017 Sep 27;15(3):166-171 [Content Brief]
[2]. Kim JS, et al. T Cell Immunoglobulin Mucin Domain (TIM)-3 Promoter Activity in a Human Mast Cell Line. Immune Netw. 2012 Oct;12(5):207-12. [Content Brief]
[3]. Gandhi AK, et al. High resolution X-ray and NMR structural study of human T-cell immunoglobulin and mucindomain containing protein-3. Sci Rep. 2018 Nov 30;8(1):17512. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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