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  4. TIM-3
  5. TIM-3/HAVCR2 Protein, Human (HEK293, Fc)

TIM-3/HAVCR2 Protein, Human (HEK293, Fc)

Cat. No.: HY-P7415
COA Handling Instructions

TIM3 Protein, Human (HEK293, Fc) is a type I transmembrane glycoprotein belonging to T Cell Immunoglobulin Mucin (TIM) family, and is involved in regulating innate and adaptive immunity.

For research use only. We do not sell to patients.

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10 μg $115 In-stock
50 μg $320 In-stock
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  • Biological Activity

  • Technical Parameters

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Description

TIM3 Protein, Human (HEK293, Fc) is a type I transmembrane glycoprotein belonging to T Cell Immunoglobulin Mucin (TIM) family, and is involved in regulating innate and adaptive immunity.

Background

T Cell Immunoglobulin Mucin 3 (TIM-3), also known as Hepatitis A virus cellular receptor 2 (HAVCR2), is an immunomodulatory molecule and upregulated in T cells by several cytokines. TIM-3 also influences mast cell function but its transcriptional regulation in mast cells has not been clarified. Human TIM-3 consists of a membrane distal variable immunoglobulin (IgV)-like domain involved in ligand interactions, a membrane proximal mucin domain and a transmembrane domain that is connected to an intracellular cytoplasmic tail involved in phosphotyrosine-dependent signaling[1][2][3].

Biological Activity

1.Immobilized TIM-3, hFc, Human at 0.5 μg/mL, the concentration of Anti-TIM3 mouse antibody (Genscript) that produces 50% optimal binding response is found to be approximately 5.0 ng/mL.
2.Immobilized Galectin-9, His, Human at 0.5 μg/mL (100 µL/well) can bind TIM-3, hFc, Human with a linear range of 0.78-6.25 μg/mL.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

Q8TDQ0 (S22-R200)

Gene ID
Molecular Construction
N-term
TIM-3 (S22-R200)
Accession # Q8TDQ0
hFc
C-term
Synonyms
rHuTIM-3, Fc Chimera; TIMD-3; CD366; HAVCR2
AA Sequence

SEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIR

Molecular Weight

60-65 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

TIM-3/HAVCR2 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
TIM-3/HAVCR2 Protein, Human (HEK293, Fc)
Cat. No.:
HY-P7415
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