TLK2 Protein, Human (Active, sf9, His, GST)

TLK2 is an important serine/threonine protein kinase with complex effects on chromatin assembly, DNA replication, transcription, repair, and chromosome segregation. It plays multiple roles by phosphorylating and stabilizing chromatin assembly factors such as ASF1A and ASF1B, preventing proteasome-mediated degradation. TLK2 Protein, Human (749a.a, sf9, His, GST) is the recombinant human-derived TLK2, expressed by Sf9 insect cells, with His, GST labeled tag.

TLK2, a serine/threonine-protein kinase, plays a crucial role in the intricate processes of chromatin assembly, potentially extending its influence to DNA replication, transcription, repair, and chromosome segregation. This kinase exhibits a multifaceted role by phosphorylating chromatin assembly factors, including ASF1A and ASF1B, and contributing to their stabilization, particularly through the prevention of proteasome-mediated degradation. By enhancing chromatin assembly, TLK2 actively participates in the maintenance of genomic integrity. Additionally, TLK2 functions as a negative regulator of amino acid starvation-induced autophagy, showcasing its involvement in cellular responses to nutritional cues. This versatile kinase, with its impact on fundamental cellular processes, emerges as a key player in the intricate orchestration of various aspects of nuclear and cellular dynamics.

Human

Sf9 insect cells

N-8*His;N-GST

Q86UE8-1 (M2-S750)

11011

Partial

MEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSAPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSAAKEATEEQSALPTLMSVMLAKPRLDTEQLAQRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQKQRKSKTNGAENETPSSGNTELKDTAPALGAHSLLRLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVS

≥ 80%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.