1. Recombinant Proteins
  2. Cytokines and Growth Factors CD Antigens Receptor Proteins
  3. TNF Superfamily Epithelial cell CD Proteins Endothelial cell CD Proteins Cytokine Receptors
  4. TNF Receptor Superfamily TNF-RI/CD120a
  5. TNF RI/TNFRSF1A Protein, Cynomolgus (HEK293, His)

TNF RI/TNFRSF1A Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P700838
Handling Instructions Technical Support

TNFRSF1A Protein exhibits a deficiency in conserved residue(s) crucial for feature annotation propagation. TNF RI/TNFRSF1A Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived TNFRSF1A protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

TNFRSF1A Protein exhibits a deficiency in conserved residue(s) crucial for feature annotation propagation. TNF RI/TNFRSF1A Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived TNFRSF1A protein, expressed by HEK293 , with C-His labeled tag.

Background

The TNFRSF1A protein is distinguished by its deficiency in conserved residue(s) essential for the propagation of feature annotation.

Biological Activity

Immobilized Cynomolgus TNFR1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the ED50 of 10.4ng/ml determined by ELISA.

Species

Cynomolgus

Source

HEK293

Tag

C-8*His

Accession

A0A7N9CVF2 (L30-T211)

Gene ID

/

Molecular Construction
N-term
TNFRSF1A (L30-T211)
Accession # A0A7N9CVF2
8*His
C-term
Protein Length

Partial

Synonyms
TNF-R1; TNF-RI; FPF; TBP1; TNF-R55; TNFAR; TNFR60; p55; p60; MS5; p55-R; TNFR1-d2
AA Sequence

LVPPLRDREKRDSVCPQGKYIHPQNNSVCCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRYYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKTLECTKLCLPQTENVKGTEDSGTT

Predicted Molecular Mass
21.6 kDa
Molecular Weight

Approximately 32-42 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Glycosylation
Yes
Purity

≥ 95%, as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TNF RI/TNFRSF1A Protein, Cynomolgus (HEK293, His)
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HY-P700838
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