TPP1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
TPP1, a lysosomal serine protease, acts as a non-specific lysosomal peptidase, displaying tripeptidyl-peptidase I activity in lysosomal protein degradation. Its function involves generating tripeptides from the breakdown products of other lysosomal proteinases, emphasizing its role in processing and hydrolyzing peptides within the lysosomal environment. Notably, TPP1 requires substrates with an unsubstituted N-terminus. TPP1 Protein, Human (HEK293, His) is the recombinant human-derived TPP1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
TPP1, a lysosomal serine protease, acts as a non-specific lysosomal peptidase, displaying tripeptidyl-peptidase I activity in lysosomal protein degradation. Its function involves generating tripeptides from the breakdown products of other lysosomal proteinases, emphasizing its role in processing and hydrolyzing peptides within the lysosomal environment. Notably, TPP1 requires substrates with an unsubstituted N-terminus. TPP1 Protein, Human (HEK293, His) is the recombinant human-derived TPP1 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
TPP1, a lysosomal serine protease, exhibits tripeptidyl-peptidase I activity, as evidenced by its involvement in lysosomal protein degradation. While its precise substrate specificity is not yet fully elucidated, TPP1 is thought to function as a non-specific lysosomal peptidase, generating tripeptides from the breakdown products produced by other lysosomal proteinases. Notably, TPP1 requires substrates with an unsubstituted N-terminus, further highlighting its role in processing and hydrolyzing peptides within the lysosomal environment.
Verified Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate Ala-Ala-Phe-AMC that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 6174.46 pmol/min/µg. (Activation description: The proenzyme needs to be activated in acid buffer for an activated form.)
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-6*His
-
Accession
AAH14863.1 (S20-P563)
-
Molecular Construction
-
N-term
-
TPP1 (S20-P563)
Accession # AAH14863.1 -
6*His
-
C-term
-
-
Protein Length
Full Length of Mature Protein (with Propeptide)
-
Synonyms
TPP1; Tripeptidyl Peptidase I; Prev. CLN2; Ceroid-Lipofuscinosis, Neuronal 2, Late Infantile (Jansky-Bielschowsky Disease); Prev. SCAR7; Spinocerebellar Ataxia, Autosomal Recessive 7; TPP-1; Lysosomal Pepstatin Insensitive Protease; LPIC; Lysosomal Pepsta
-
AA Sequence
SYSPEPDQRRTLPPGWVSLGRADPEEELSLTFALRQQNVERLSELVQAVSDPSSPQYGKYLTLENVADLVRPSPLTLHTVQKWLLAAGAQKCHSVITQDFLTCWLSIRQAELLLPGAEFHHYVGGPTETHVVRSPHPYQLPQALAPHVDFVGGLHRFPPTSSLRQRPEPQVTGTVGLHLGVTPSVIRKRYNLTSQDVGSGTSNNSQACAQFLEQYFHDSDLAQFMRLFGGNFAHQASVARVVGQQGRGRAGIEASLDVQYLMSAGANISTWVYSSPGRHEGQEPFLQWLMLLSNESALPHVHTVSYGDDEDSLSSAYIQRVNTELMKAAARGLTLLFASGDSGAGCWSVSGRHEFRPTFPASSPYVTTVGGTSFQEPFLITNEIVDYISGGGFSNVFPRPSYQEEAVTKFLSSSPHLPPSSYFNASGRAYPDVAALSDGYWVVSNRVPIPWVSGTSASTPVFGGILSLINEHRILSGRPPLGFLNPRLYQQHGAGLFDVTRGCHESCLDEEVEGQGFCSGPGWDPVTGWGTPNFPALLKTLLNP
-
Predicted Molecular Mass
60.4 kDa
-
Molecular Weight
Approximately 74 kDa, based on SDS-PAGE under reducing conditions.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM CaCl2, 10% glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
-
Data Sheet (239 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
