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  3. TRPC3 Protein, Human (HEK293, His-MBP, Flag)

TRPC3 Protein, Human (HEK293, His-MBP, Flag) is the recombinant human-derived TRPC3, expressed by HEK293 , with N-His-MBP, C-Flag labeled tag. ,

For research use only. We do not sell to patients.

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50 μg In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

TRPC3 Protein, Human (HEK293, His-MBP, Flag) is the recombinant human-derived TRPC3, expressed by HEK293 , with N-His-MBP, C-Flag labeled tag. ,

Background

TRPC3 forms a receptor-activated non-selective calcium-permeant cation channel involved in intracellular calcium signaling. Key features include: 1) receptor activation; 2) non-selective cation channel properties; 3) calcium permeability. Supporting evidence comes from: PubMed:29726814, PubMed:30139744, PubMed:35051376, PubMed:9417057, PubMed:9930701, PubMed:10611319. by similar: Other TRPC family members may share similar functions.

Species

Human

Source

HEK293

Tag

N-His;C-Flag;N-MBP

Accession

Q13507 (R2-E836)

Gene ID

7222

Protein Length

Partial

Synonyms
TRP3
Predicted Molecular Mass
139.7 kDa
Molecular Weight

Approximately 130 kDa

Purity

≥ 90%, as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 1*PBS, 1 mM DDT, 0.05% (w/v) LMNG, 0.01% (w/v) CHS, 0.1 mg/mL POPC:POPE:POPG (3:1:1).

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

TRPC3 Protein, Human (HEK293, His-MBP, Flag) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TRPC3 Protein, Human (HEK293, His-MBP, Flag)

 

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Product Name:
TRPC3 Protein, Human (HEK293, His-MBP, Flag)
Cat. No.:
HY-P705532
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