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Tryptophan Hydroxylase 1/TPH-1 Protein, Human (His)

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The tryptophan hydroxylase 1 (TPH-1) protein is key to serotonin biosynthesis, catalyzing the rate-limiting step: the oxidation of L-tryptophan to 5-hydroxy-L-tryptophan. This enzyme activity is critical for the synthesis of serotonin, a neurotransmitter that affects mood, sleep, and appetite. Tryptophan Hydroxylase 1/TPH-1 Protein, Human (His) is the recombinant human-derived Tryptophan Hydroxylase 1/TPH-1 protein, expressed by E. coli , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The tryptophan hydroxylase 1 (TPH-1) protein is key to serotonin biosynthesis, catalyzing the rate-limiting step: the oxidation of L-tryptophan to 5-hydroxy-L-tryptophan. This enzyme activity is critical for the synthesis of serotonin, a neurotransmitter that affects mood, sleep, and appetite. Tryptophan Hydroxylase 1/TPH-1 Protein, Human (His) is the recombinant human-derived Tryptophan Hydroxylase 1/TPH-1 protein, expressed by E. coli , with N-His labeled tag.

Background

Tryptophan Hydroxylase 1 (TPH-1) Protein plays a pivotal role in serotonin biosynthesis by catalyzing the rate-determining step, which involves the oxidation of L-tryptophan to 5-hydroxy-L-tryptophan. This enzymatic activity is crucial for the synthesis of serotonin, a neurotransmitter with essential roles in various physiological processes, including mood regulation, sleep, and appetite. TPH-1's ability to convert L-tryptophan to 5-hydroxy-L-tryptophan is a key regulatory step in the serotonin pathway, highlighting its significance in neurotransmitter production and neurotransmission.

Verified Bioactivity

Measured by its ability to catalyze Trp hydroxylation to 5- HTP.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-His
  • Accession

    P17752 (I2-I444)

  • Gene ID
    7166  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • TPH-1 (I2-I444)
      Accession # P17752
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    TPH1; Tryptophan 5-Monooxygenase; Prev. TPRH; TRPH; Prev. TPH; Indoleacetic Acid-5-Hydroxylase; Tryptophan Hydroxylase (Tryptophan 5-Monooxygenase); L-Tryptophan Hydroxylase; Tryptophan 5-Monooxygenase 1; TPH1 Protein; Tryptophan Hydroxylase 1; Tryptophan

  • AA Sequence

    IEDNKENKDHSLERGRASLIFSLKNEVGGLIKALKIFQEKHVNLLHIESRKSKRRNSEFEIFVDCDINREQLNDIFHLLKSHTNVLSVNLPDNFTLKEDGMETVPWFPKKISDLDHCANRVLMYGSELDADHPGFKDNVYRKRRKYFADLAMNYKHGDPIPKVEFTEEEIKTWGTVFQELNKLYPTHACREYLKNLPLLSKYCGYREDNIPQLEDVSNFLKERTGFSIRPVAGYLSPRDFLSGLAFRVFHCTQYVRHSSDPFYTPEPDTCHELLGHVPLLAEPSFAQFSQEIGLASLGASEEAVQKLATCYFFTVEFGLCKQDGQLRVFGAGLLSSISELKHALSGHAKVKPFDPKITCKQECLITTFQDVYFVSESFEDAKEKMREFTKTIKRPFGVKYNPYTRSIQILKDTKSITSAMNELQHDLDVVSDALAKVSRKPSI

  • Predicted Molecular Mass

    52.7 kDa

  • Molecular Weight

    Approximately 48 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 200 mM NaCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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