Tryptophan Hydroxylase 1/TPH-1 Protein, Human (His)

The tryptophan hydroxylase 1 (TPH-1) protein is key to serotonin biosynthesis, catalyzing the rate-limiting step: the oxidation of L-tryptophan to 5-hydroxy-L-tryptophan. This enzyme activity is critical for the synthesis of serotonin, a neurotransmitter that affects mood, sleep, and appetite. Tryptophan Hydroxylase 1/TPH-1 Protein, Human (His) is the recombinant human-derived Tryptophan Hydroxylase 1/TPH-1 protein, expressed by E. coli , with N-His labeled tag.

Tryptophan Hydroxylase 1 (TPH-1) Protein plays a pivotal role in serotonin biosynthesis by catalyzing the rate-determining step, which involves the oxidation of L-tryptophan to 5-hydroxy-L-tryptophan. This enzymatic activity is crucial for the synthesis of serotonin, a neurotransmitter with essential roles in various physiological processes, including mood regulation, sleep, and appetite. TPH-1's ability to convert L-tryptophan to 5-hydroxy-L-tryptophan is a key regulatory step in the serotonin pathway, highlighting its significance in neurotransmitter production and neurotransmission.

Measured by its ability to catalyze Trp hydroxylation to 5- HTP.

Human

E. coli

N-His

P17752 (I2-I444)

7166  [NCBI]

Partial

IEDNKENKDHSLERGRASLIFSLKNEVGGLIKALKIFQEKHVNLLHIESRKSKRRNSEFEIFVDCDINREQLNDIFHLLKSHTNVLSVNLPDNFTLKEDGMETVPWFPKKISDLDHCANRVLMYGSELDADHPGFKDNVYRKRRKYFADLAMNYKHGDPIPKVEFTEEEIKTWGTVFQELNKLYPTHACREYLKNLPLLSKYCGYREDNIPQLEDVSNFLKERTGFSIRPVAGYLSPRDFLSGLAFRVFHCTQYVRHSSDPFYTPEPDTCHELLGHVPLLAEPSFAQFSQEIGLASLGASEEAVQKLATCYFFTVEFGLCKQDGQLRVFGAGLLSSISELKHALSGHAKVKPFDPKITCKQECLITTFQDVYFVSESFEDAKEKMREFTKTIKRPFGVKYNPYTRSIQILKDTKSITSAMNELQHDLDVVSDALAKVSRKPSI

Approximately 48 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 200 mM NaCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.