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USP35 Protein, Human (Active, sf9, GST )

USP35 Protein, Human (Active, sf9, GST ) is the recombinant human-derived USP35 protein, expressed by Sf9 insect cells, with N-GST tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • USP35 Protein, Human (Active, sf9, GST ) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

USP35 Protein, Human (Active, sf9, GST ) is the recombinant human-derived USP35 protein, expressed by Sf9 insect cells, with N-GST tag.

Background

USP35 is a deubiquitinase involved in various cellular processes, including cell cycle regulation, mitophagy, and endoplasmic reticulum stress. It inhibits TNFalpha-induced NF-kappa-B activation by stabilizing TNIP2 protein through deubiquitination. During mitosis, USP35 plays an essential role by deubiquitinating and regulating Aurora B/AURKB protein levels. Additionally, it regulates the protein levels of key chromosomal passenger complex (CPC) components such as survivin/BIRC5 and Borealin/CDCA8 by enhancing their stability. USP35 also controls mitochondrial degradation through the autophagy process termed mitophagy. by similar: Other studies suggest USP35 may participate in additional, yet unidentified cellular regulatory pathways.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-GST
  • Accession

    Q9P2H5-1 (M395-F1018)

  • Gene ID
    57558
  • Molecular Construction
    • N-term
    • GST
    • (M395-F1018)
      Accession # Q9P2H5-1
    • C-term
  • Protein Length

    Partial

  • Synonyms

    USP35; Ubiquitin Specific Peptidase 35; KIAA1372; Ubiquitin-Specific-Processing Protease 35; Ubiquitin Carboxyl-Terminal Hydrolase 35; Ubiquitin Specific Protease 35; Deubiquitinating Enzyme 35; Ubiquitin Thioesterase 35; Ubiquitin Carboxyl-Terminal Hydro

  • AA Sequence

    MEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGKIGLINLGNTCYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAEKVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGRGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRVRTEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFDEDKDEDEGSPGGCNPAGGNGGDFHRLVF

  • Molecular Weight

    Approximately 105 kDa, based on SDS-PAGE under reducing conditions.

  • Structure/Form

    Monomer

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • USP35 Protein, Human (Active, sf9, GST ) Product Image
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  • USP35 Protein, Human (Active, sf9, GST ) Product Image