USP5 Protein, Human (His)
Based on 1 Customer Validation
USP5 protein selectively cleaves branched multiubiquitin polymers, favoring 'Lys-48'-linked polyubiquitin disassembly. It exhibits lower affinity for linear and 'Lys-63'-linked polyubiquitin. USP5 knockdown leads to p53/TP53 accumulation, enhancing p53/TP53 transcriptional activity, as unanchored polyubiquitin competes with ubiquitinated p53/TP53 for proteasomal recognition, unlike MDM2. USP5 Protein, Human (His) is the recombinant human-derived USP5 protein, expressed by E. coli, with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
USP5 protein selectively cleaves branched multiubiquitin polymers, favoring 'Lys-48'-linked polyubiquitin disassembly. It exhibits lower affinity for linear and 'Lys-63'-linked polyubiquitin. USP5 knockdown leads to p53/TP53 accumulation, enhancing p53/TP53 transcriptional activity, as unanchored polyubiquitin competes with ubiquitinated p53/TP53 for proteasomal recognition, unlike MDM2. USP5 Protein, Human (His) is the recombinant human-derived USP5 protein, expressed by E. coli, with N-His labeled tag.
USP5, a deubiquitinating enzyme, demonstrates a marked preference for cleaving both linear and branched multiubiquitin polymers, with a particular affinity for branched structures. It is actively involved in the disassembly of unanchored 'Lys-48'-linked polyubiquitin chains. While USP5 binds linear and 'Lys-63'-linked polyubiquitin with a comparatively lower affinity, its knockdown results in the accumulation of p53/TP53 and an elevation in p53/TP53 transcriptional activity. This effect is attributed to the ability of the unanchored polyubiquitin accumulating in the absence of USP5 to compete with ubiquitinated p53/TP53, but not with MDM2, for recognition by the proteasome.
The fundamental role of USP5 is specific removal of ubiquitin from substrates. USP5 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM. The signal of which can be quickly and reliably captured using a microplate reader.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His
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Accession
P45974-1 (A2-S858)
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Gene ID8078
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
USP5; Ubiquitin Specific Peptidase 5; Isopeptidase T; IsoT; Ubiquitin‑Specific‑Processing Protease 5; Ubiquitin Carboxyl‑Terminal Hydrolase 5; Deubiquitinating Enzyme 5; Ubiquitin Thioesterase 5; Ubiquitin‑Specific Protease‑5 (Ubiquitin Isopeptidase T); U
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AA Sequence
AELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGERVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVAS
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Predicted Molecular Mass
97.8 kDa
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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