USP6 Protein, Human (sf9, GST)
Based on 1 Customer Validation
USP6 protein is an ATP-independent isopeptidase with unique deubiquitinase activity that can cleave the C-terminus of ubiquitin and catalyze its own deubiquitination. Notably, it coordinates ARF6 plasma membrane localization and selectively affects ARF6-dependent endocytic protein trafficking. USP6 Protein, Human (sf9, GST) is the recombinant human-derived USP6 protein, expressed by sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
USP6 protein is an ATP-independent isopeptidase with unique deubiquitinase activity that can cleave the C-terminus of ubiquitin and catalyze its own deubiquitination. Notably, it coordinates ARF6 plasma membrane localization and selectively affects ARF6-dependent endocytic protein trafficking. USP6 Protein, Human (sf9, GST) is the recombinant human-derived USP6 protein, expressed by sf9 insect cells , with N-GST labeled tag.
Background
USP6, an ATP-independent isopeptidase, possesses distinctive deubiquitinase activity, cleaving at the C-terminus of the ubiquitin moiety and catalyzing its own deubiquitination. In vitro, isoform 2 exhibits deubiquitinating activity, contrasting with isoform 3. Notably, USP6 plays a crucial role in orchestrating plasma membrane localization of ARF6 and selectively modulating ARF6-dependent endocytic protein trafficking. Its functional significance extends to tumorigenesis initiation, as it induces the production of matrix metalloproteinases through NF-kappa-B activation. USP6's multifaceted activity highlights its pivotal role in cellular processes, positioning it as a key player in protein regulation, membrane dynamics, and the intricate balance involved in tumorigenesis.
Verified Bioactivity
The fundamental role of USP6 is specific removal of ubiquitin from substrates. USP6 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader. At a concentration of 0.03 nM, USP6 reacts with the Ub-Rho110 substrate. The signal window after half an hour of reaction can be greater than or equal to 10.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
P35125 (K529-Q1406)
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Gene ID9098
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Molecular Construction
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N-term
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GST
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USP6 (K529-Q1406)
Accession # P35125 -
C-term
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Protein Length
Partial
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Synonyms
USP6; Ubiquitin Specific Peptidase 6; HRP1; TRESMCR; Ubiquitin Carboxyl-Terminal Hydrolase 6; Tre-2; TRE17; Tre2; Ubiquitin Specific Peptidase 6 (Tre-2 Oncogene); Ubiquitin Specific Protease 6 (Tre-2 Oncogene); Ubiquitin-Specific-Processing Protease 6; De
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AA Sequence
KGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKSVAPLKLRRTIAKYAPKFDGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIIVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMDLEITVIKLDGTTPVRYGLRLNMDEKYTGLKKQLRDLCGLNSEQILLAEVHDSNIKNFPQDNQKVQLSVSGFLCAFEIPVPSSPISASSPTQIDFSSSPSTNGMFTLTTNGDLPKPIFIPNGMPNTVVPCGTEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSPQENRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSWLARPLPPQEASIHAQDRDNCMGYQYPFTLRVVQKDGNSCAWCPQYRFCRGCKIDCGEDRAFIGNAYIAVDWHPTALHLRYQTSQERVVDKHESVEQSRRAQAEPINLDSCLRAFTSEEELGESEMYYCSKCKTHCLATKKLDLWRLPPFLIIHLKRFQFVNDQWIKSQKIVRFLRESFDPSAFLVPRDPALCQHKPLTPQGDELSKPRILAREVKKVDAQSSAGKEDMLLSKSPSSLSANISSSPKGSPSSSRKSGTSCPSSKNSSPNSSPRTLGRSKGRLRLPQIGSKNKPSSSKKNLDASKENGAGQICELADALSRGHMRGGSQPELVTPQDHEVALANGFLYEHEACGNGCGDGYSNGQLGNHSEEDSTDDQREDTHIKPIYNLYAISCHSGILSGGHYITYAKNPNCKWYCYNDSSCEELHPDEIDTDSAYILFYEQQGIDYAQFLPKIDGKKMADTSSTDEDSESDYEKYSMLQ
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Predicted Molecular Mass
125.1 KDa
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 1 mM DTT, 20% glycerol.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (241 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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