VRK3 Protein, Human (sf9, His, GST)
Despite its inactive kinase state, VRK3 protein crucially regulates ERK activity by enhancing the phosphatase activity of DUSP3. The interaction with DUSP3 is pivotal as it dephosphorylates and inactivates ERK in the nucleus. VRK3, also interacting with the small GTPase RAN, forms a network of molecular interactions, positioning it as a key modulator in signaling pathways controlling ERK activity and nuclear cellular processes. VRK3 Protein, Human (sf9, His, GST) is the recombinant human-derived VRK3 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Despite its inactive kinase state, VRK3 protein crucially regulates ERK activity by enhancing the phosphatase activity of DUSP3. The interaction with DUSP3 is pivotal as it dephosphorylates and inactivates ERK in the nucleus. VRK3, also interacting with the small GTPase RAN, forms a network of molecular interactions, positioning it as a key modulator in signaling pathways controlling ERK activity and nuclear cellular processes. VRK3 Protein, Human (sf9, His, GST) is the recombinant human-derived VRK3 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
Background
The VRK3 protein, despite its inactive kinase state, plays a crucial role in the regulation of ERK activity by promoting the phosphatase activity of DUSP3. This interaction is pivotal, as DUSP3 specifically dephosphorylates and inactivates ERK within the nucleus. In addition to its association with DUSP3, VRK3 interacts with the small GTPase RAN, indicating a network of molecular interactions. Through these associations, VRK3 emerges as a key modulator in the intricate signaling pathways involved in the control of ERK activity and cellular processes within the nucleus.
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag N-8*His;N-GST
-
Accession
Q8IV63 (I2-P474)
-
Gene ID51231
-
Molecular Construction
-
N-term
-
8*His-GST
-
VRK3 (I2-P474)
Accession # Q8IV63 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
VRK3; VRK Serine/Threonine Kinase 3; Serine/Threonine-Protein Kinase VRK3; Vaccinia Related Kinase 3; Vaccinia-Related Kinase 3; Inactive Serine/Threonine-Protein Kinase VRK3; Serine/Threonine-Protein Pseudokinase VRK3
-
AA Sequence
ISFCPDCGKSIQAAFKFCPYCGNSLPVEEHVGSQTFVNPHVSSFQGSKRGLNSSFETSPKKVKWSSTVTSPRLSLFSDGDSSESEDTLSSSERSKGSGSRPPTPKSSPQKTRKSPQVTRGSPQKTSCSPQKTRQSPQTLKRSRVTTSLEALPTGTVLTDKSGRQWKLKSFQTRDNQGILYEAAPTSTLTCDSGPQKQKFSLKLDAKDGRLFNEQNFFQRAAKPLQVNKWKKLYSTPLLAIPTCMGFGVHQDKYRFLVLPSLGRSLQSALDVSPKHVLSERSVLQVACRLLDALEFLHENEYVHGNVTAENIFVDPEDQSQVTLAGYGFAFRYCPSGKHVAYVEGSRSPHEGDLEFISMDLHKGCGPSRRSDLQSLGYCMLKWLYGFLPWTNCLPNTEDIMKQKQKFVDKPGPFVGPCGHWIRPSETLQKYLKVVMALTYEEKPPYAMLRNNLEALLQDLRVSPYDPIGLPMVP
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
