VRK3 Protein, Human (sf9, His, GST)

Despite its inactive kinase state, VRK3 protein crucially regulates ERK activity by enhancing the phosphatase activity of DUSP3. The interaction with DUSP3 is pivotal as it dephosphorylates and inactivates ERK in the nucleus. VRK3, also interacting with the small GTPase RAN, forms a network of molecular interactions, positioning it as a key modulator in signaling pathways controlling ERK activity and nuclear cellular processes. VRK3 Protein, Human (sf9, His, GST) is the recombinant human-derived VRK3 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.

The VRK3 protein, despite its inactive kinase state, plays a crucial role in the regulation of ERK activity by promoting the phosphatase activity of DUSP3. This interaction is pivotal, as DUSP3 specifically dephosphorylates and inactivates ERK within the nucleus. In addition to its association with DUSP3, VRK3 interacts with the small GTPase RAN, indicating a network of molecular interactions. Through these associations, VRK3 emerges as a key modulator in the intricate signaling pathways involved in the control of ERK activity and cellular processes within the nucleus.

Human

Sf9 insect cells

N-8*His;N-GST

Q8IV63 (I2-P474)

51231

Partial

ISFCPDCGKSIQAAFKFCPYCGNSLPVEEHVGSQTFVNPHVSSFQGSKRGLNSSFETSPKKVKWSSTVTSPRLSLFSDGDSSESEDTLSSSERSKGSGSRPPTPKSSPQKTRKSPQVTRGSPQKTSCSPQKTRQSPQTLKRSRVTTSLEALPTGTVLTDKSGRQWKLKSFQTRDNQGILYEAAPTSTLTCDSGPQKQKFSLKLDAKDGRLFNEQNFFQRAAKPLQVNKWKKLYSTPLLAIPTCMGFGVHQDKYRFLVLPSLGRSLQSALDVSPKHVLSERSVLQVACRLLDALEFLHENEYVHGNVTAENIFVDPEDQSQVTLAGYGFAFRYCPSGKHVAYVEGSRSPHEGDLEFISMDLHKGCGPSRRSDLQSLGYCMLKWLYGFLPWTNCLPNTEDIMKQKQKFVDKPGPFVGPCGHWIRPSETLQKYLKVVMALTYEEKPPYAMLRNNLEALLQDLRVSPYDPIGLPMVP

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.